IL-1RL2 Protein, Human (HEK293, His)

IL-1RL2 Protein, serving as a receptor for interleukin-36 (IL36A, IL36B, and IL36G), forms a complex with the coreceptor IL1RAP, constituting the interleukin-36 receptor. This complex activates NF-kappa-B, MAPK, and other pathways, resembling the IL-1 system. IL-1RL2 is implicated in local inflammatory responses at epithelial barriers, particularly contributing to skin inflammatory reactions by inducing the IL-23/IL-17/IL-22 pathway. IL-1RL2 Protein, Human (HEK293, His) is the recombinant human-derived IL-1RL2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of IL-1RL2 Protein, Human (HEK293, His) is 318 a.a., with molecular weight of ~60.0 kDa.

IL-1RL2, a receptor for interleukin-36 (IL36A, IL36B, and IL36G), engages in complex signaling events upon binding to interleukin-36. Through association with the coreceptor IL1RAP, the interleukin-36 receptor complex is formed, orchestrating interleukin-36-dependent activation of NF-kappa-B, MAPK, and other pathways. This signaling system, akin to the IL-1 system, is implicated in local inflammatory responses within epithelial barriers, particularly contributing to skin inflammatory reactions by inducing the IL-23/IL-17/IL-22 pathway.

Immobilized IL-1F5 (HY-P71965) at 1 μg/mL (100 μL/well) can bind Human IL-1RL2 Protein. The ED₅₀ for this effect is 2.591 μg/mL.

Human

HEK293

C-6*His

Q9HB29 (D20-Y337)

8808  [NCBI]

DGCKDIFMKNEILSASQPFAFNCTFPPITSGEVSVTWYKNSSKIPVSKIIQSRIHQDETWILFLPMEWGDSGVYQCVIKGRDSCHRIHVNLTVFEKHWCDTSIGGLPNLSDEYKQILHLGKDDSLTCHLHFPKSCVLGPIKWYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNGITVSITERAGYGGSVPKIIYPKNHSIEVQLGTTLIVDCNVTDTKDNTNLRCWRVNNTLVDDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPAPDFRAY

Approximately 60.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.