IL-2 Protein, Canine (C147S)

IL-2 Protein, produced by activated T-cells, NK cells, and helper T-cells, is crucial for immune response and tolerance. It binds to receptor complexes, activating signaling pathways. IL-2 Protein acts as a growth factor, enhances NK-cell activity, promotes B-cell proliferation, and regulates regulatory T-cells. It also participates in differentiating effector T-cell subsets and memory CD8-positive T-cells. IL-2 Protein, Canine (C147S) is the recombinant canine-derived IL-2 protein, expressed by E. coli , with tag free and C147S mutation.

IL-2 Protein, produced by activated CD4-positive helper T-cells and to a lesser extent activated CD8-positive T-cells and natural killer (NK) cells, plays vital roles in the immune response and tolerance. It binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122, and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG), leading to downstream signaling through phosphorylation of JAK1 and JAK3. This activation triggers various pathways, including STAT, phosphoinositide-3-kinase/PI3K, and mitogen-activated protein kinase/MAPK pathways. IL-2 Protein acts as a T-cell growth factor, enhancing NK-cell cytolytic activity, promoting the proliferation of activated B-cells, and stimulating immunoglobulin production. It also plays a crucial role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are essential for immune tolerance. Additionally, IL-2 Protein participates in the differentiation and homeostasis of effector T-cell subsets, such as Th1, Th2, Th17, and memory CD8-positive T-cells.

1.Measured by its binding ability in a functional ELISA.Immobilized recombinant Canine IL2 at 10 μg/mL can bind recombinant human IL2Ra-Fc with a linear range of 0.3125-20 μg/mL.
2.Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.3569 ng/mL, corresponding to a specific activity is 2.8×10⁶ units/mg.

Canine

E. coli

Tag Free

Q29416 (A21-T155, C147S)

403989  [NCBI]

APITSSSTKETEQQMEQLLLDLQLLLNGVNNYENPQLSRMLTFKFYTPKKATEFTHLQCLAEELKNLEEVLGLPQSKNVHLTDTKELISNMNVTLLKLKGSETSYNCEYDDETATITEFLNKWITFSQSIFSTLT

Approximately 15-16 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.