IL-2 Protein, Rat (HEK293, His)

IL-2 Protein, produced by activated T-cells, NK cells, and helper T-cells, is crucial for immune response and tolerance. It binds to receptor complexes, activating downstream signaling pathways. IL-2 Protein acts as a growth factor, enhances NK-cell activity, promotes B-cell proliferation, and regulates regulatory T-cells. It also participates in differentiating effector T-cell subsets and memory CD8-positive T-cells. IL-2 Protein, Rat (HEK293, His) is the recombinant rat-derived IL-2 protein, expressed by HEK293 , with C-6*His labeled tag.

IL-2, a cytokine primarily produced by activated CD4-positive helper T-cells, and to a lesser extent by activated CD8-positive T-cells and natural killer (NK) cells, plays pivotal roles in immune response and tolerance. It binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122, and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). The interaction triggers oligomerization and conformational changes in the IL-2R subunits, initiating downstream signaling that begins with the phosphorylation of JAK1 and JAK3. Consequently, JAK1 and JAK3 phosphorylate the receptor, creating a docking site for the phosphorylation of substrates, including STAT5, activating pathways such as STAT, phosphoinositide-3-kinase/PI3K, and mitogen-activated protein kinase/MAPK. IL-2 functions as a T-cell growth factor, enhances NK-cell cytolytic activity, and fosters robust proliferation of activated B-cells, leading to increased immunoglobulin production. Crucially, IL-2 plays a pivotal role in regulating the adaptive immune system by governing the survival and proliferation of regulatory T-cells, crucial for immune tolerance maintenance. Furthermore, it participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17, as well as memory CD8-positive T-cells.

Rat

HEK293

C-6*His

P17108 (A21-Q155)

116562  [NCBI]

APTSSPAKETQQHLEQLLLDLQVLLRGIDNYKNLKLPMMLTFKFYLPKQATELKHLQCLENELGALQRVLDLTQSKSFHLEDAGNFISNIRVTVVKLKGSENKFECQFDDEPATVVEFLRRWIAICQSIISTMTQ

14-16 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 5 mM NaH₂PO₄, 5 mM Citric acid, 150 mM NaCl, pH 4.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.