IL-2R beta & IL-2R gamma Heterodimer Protein, Human (HEK293, Fc)

IL-2R beta (CD122) and IL-2Rγ (CD132), type Ⅰ cytokine receptors expressed on T lymphocytes, are receptors for IL-2 (K_d: 1 nM for IL-2R beta approximately)^[1]. IL-2R beta mediates T cell immune responses. IL-2Rγ mediates inflammatory response^[2]
IL-2Rβ and IL-2Rγ heterodimer binds to IL-2 with intermediate affinity^[6]. IL-2R beta & IL-2R gamma Heterodimer Protein, Human (HEK293, Fc) is a recombinant human IL-2R beta (A27-D239) and IL-2R gamma (L23-A262) heterodimer complex, with a C-Terminal hFc tag, which is produced in HEK293 cells.

IL-2R beta and IL-2R gama are both type I cytokine receptors, and belongs to Type 4 subfamily. IL-2R beta/gama heterodimer has intermediate affinity for IL-2. IL-2R beta/gama heterodimer complex transduces a signal when IL-2 concentrations are relatively high.
IL-2R beta is a key component of the IL-15 receptor. IL-2R beta is broadly expressed in spleen, blood, and lymph node, such as B and T lymphocytes^[1][3].IL-2R gama is expressed in leucocyte subsets. IL-2R gama is involved in inflammatory response, and mediates activation of the cells^[6]
IL-2R beta cytoplasmic domain heterodimerizes with IL-2 and leads to the activation of signaling pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAP kinase, and the JAK-STAT pathways^[4]. IL-2R beta binds IL-2 with intermediate affinity. IL-2R beta mediates IL-2 internalization and signal transduction, such as cell proliferation or differentiation^[5]. IL-2R beta interacts with IL-2 and increases the proportion of CD4+ T lymphocytes^[1]. IL-2R stimulates T cell proliferation and activating lymphokine-activated killer cells^[2].
IL-2R beta mediates T cell immune responses, and also mediates endocytosis, as well as transducing the mitogenic signals of IL-2.

Immobilized Human IL-15RA&IL-15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC₅₀ of <72.0ng/ml determined by ELISA.

Human

HEK293

C-hFc

P14784 (A27-T240)&P31785 (L23-A262)

3560  [NCBI]&3561  [NCBI]

65-70 kDa & 80-100 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS(pH 7.4). Normally 8% Trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Xiujuan Zhou, et al. Interleukin-2 (IL-2) Interacts With IL-2 Receptor Beta (IL-2Rβ): Its Potential to Enhance the Proliferation of CD4+ T Lymphocytes in Flounder ( Paralichthys olivaceus). Front Immunol. 2020 Sep 9;11:531785.  [Content Brief]

  [2]. R N Bamfordm, et al. The interleukin (IL) 2 receptor beta chain is shared by IL-2 and a cytokine, provisionally designated IL-T, that stimulates T-cell proliferation and the induction of lymphokine-activated killer cells. Proc Natl Acad Sci U S A.1994 May 24;91(11):4940-4.  [Content Brief]

  [3]. Xiujuan Zhou, et al. Immunological characteristics of Interleukin-2 receptor subunit beta (IL-2Rβ) in flounder (Paralichtlys olivaceus): Implication for IL-2R function. Fish Shellfish Immunol. 2019 Oct;93:641-651.  [Content Brief]

  [4]. AkiraSakai,etal.Theroleoftumor-associatedmacrophagesonserumsolubleIL-2RlevelsinB-celllymphomas.JClinExpHematop.2014;54(1):49-57.  [Content Brief]

  [5]. MAllouche,etal.Interleukin2receptors.LeukRes.1990;14(8):699-703.  [Content Brief]

  [6]. S Hodge, et al. Surface and intracellular interleukin-2 receptor expression on various resting and activated populations involved in cell-mediated immunity in human peripheral blood. Scand J Immunol. 2000 Jan;51(1):67-72.  [Content Brief]