IL-4 Protein, Human (CHO)

IL-4 Protein, Human (CHO) is a CHO cell derived, potent lymphoid cell growth factor, which stimulates the growth and survivability of B-cells and T-cells.

Interleukin-4 (IL-4) is a cytokine that plays an important role in regulating inflammation and immune responses. It induces the differentiation of naïve helper T cells to Th2 cells. This cytokine binds the IL-4 receptor that also binds another cytokine interleukin 13 (IL-13), which may explain the overlapping functions of IL-4 and IL-13. IL-4, originally designated as B-cell growth factor-1 (BSFl), is described in the supernatant of activated EL-4 thymoma cells as a factor that can co-stimulate B-cells activated with submitogenic concentrations of anti-IgM^[1].

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED ₅₀ for this effect is <0.25 ng/mL, corresponding to a specific activity is >4 × 10⁶units/mg.

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  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 0.08343 ng/mL, corresponding to a specific activity is 1.99 × 10⁷units/mg.

Human

CHO

Tag Free

P05112-1 (H25-S153)

3565  [NCBI]

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Approximately 17-21 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Le HV, et al. Isolation and characterization of multiple variants of recombinant human interleukin 4 expressed in mammalian cells. J Biol Chem. 1988 Aug 5;263(22):10817-23.  [Content Brief]