Insulin Protein, Human (P.pastoris)

Insulin is an important hormone in glucose regulation, reducing blood glucose concentrations by increasing cellular permeability to simple sugars, amino acids, and fatty acids. Its multiple effects include accelerating glycolysis, promoting pentose phosphate cycle, and promoting hepatic glycogen synthesis. Insulin Protein, Human (P.pastoris) is a recombinant protein dimer complex containing human-derived Insulin protein, expressed by P. pastoris , with tag free. Insulin Protein, Human (P.pastoris), has molecular weight of ~6 kDa.

Insulin, a pivotal hormone in glucose regulation, effectively reduces blood glucose concentration by enhancing cellular permeability to monosaccharides, amino acids, and fatty acids. Its multifaceted actions include the acceleration of glycolysis, promotion of the pentose phosphate cycle, and facilitation of glycogen synthesis in the liver. The insulin molecule itself is composed of two subunits, a B chain and an A chain, intricately linked by two disulfide bonds. These structural features, as documented in recent studies, underpin the hormone's essential role in orchestrating metabolic processes critical for maintaining glucose homeostasis.

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells and the ED₅₀ is typically 0.2-1 µg/mL.

Human

P. pastoris

Tag Free

P01308-1 (F25-K53&G90-N110)

3630  [NCBI]

Approximately 6 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.