1. Recombinant Proteins
  2. Receptor Proteins Biotinylated Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Integrin
  5. Integrin alpha 5 beta 1
  6. Integrin alpha 5 beta 1 Protein, Human (Biotinylated, HEK293, His-Avi)

Integrin alpha 5 beta 1 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P700755
Handling Instructions

ITGA5, a versatile receptor, interacts with diverse ligands such as fibronectin, fibrinogen, PLA2G2A, fibrillin-1 (FBN1), IL1B, Human metapneumovirus, and soluble CD40LG. Recognizing the R-G-D sequence, ITGA5 mediates cell adhesion through distinct binding sites. Notably, it binds PLA2G2A at a separate site, inducing conformational changes enhancing ligand binding. ITGA5's role in IL1B signaling, microbial infection, and CD40/CD40LG signaling highlights its multifunctionality in cellular processes and signaling pathways. Integrin alpha 5 beta 1 Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived Integrin alpha 5 beta 1 protein, expressed by HEK293, with C-Avi, C-His labeled tag. Integrin alpha 5 beta 1 Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of 110-140 kDa.

For research use only. We do not sell to patients.

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Description

ITGA5, a versatile receptor, interacts with diverse ligands such as fibronectin, fibrinogen, PLA2G2A, fibrillin-1 (FBN1), IL1B, Human metapneumovirus, and soluble CD40LG. Recognizing the R-G-D sequence, ITGA5 mediates cell adhesion through distinct binding sites. Notably, it binds PLA2G2A at a separate site, inducing conformational changes enhancing ligand binding. ITGA5's role in IL1B signaling, microbial infection, and CD40/CD40LG signaling highlights its multifunctionality in cellular processes and signaling pathways. Integrin alpha 5 beta 1 Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived Integrin alpha 5 beta 1 protein, expressed by HEK293, with C-Avi, C-His labeled tag. Integrin alpha 5 beta 1 Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of 110-140 kDa.

Background

The Integrin alpha-5/beta-1 protein (ITGA5:ITGB1) functions as a versatile receptor with diverse ligand interactions. Recognizing the R-G-D sequence in its ligands, ITGA5:ITGB1 serves as a receptor for fibronectin and fibrinogen, mediating cell adhesion through distinct binding sites. Notably, it binds to PLA2G2A at a site separate from its classical ligand-binding site, inducing conformational changes that enhance ligand binding. Additionally, ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1), facilitating R-G-D-dependent cell adhesion. It is also a receptor for fibronectin (FN1), enabling R-G-D-dependent cell adhesion to FN1. Furthermore, ITGA5:ITGB1 serves as a receptor for IL1B, playing a crucial role in IL1B signaling. In the context of microbial infection, ITGA5:ITGB1 acts as a receptor for Human metapneumovirus, highlighting its involvement in pathogen recognition. Moreover, ITGA5:ITGB3 acts as a receptor for soluble CD40LG, playing a vital role in CD40/CD40LG signaling. This broad spectrum of ligand interactions underscores the multifunctionality of ITGA5:ITGB1 in cellular processes and signaling pathways.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

P08648 (F42-Y995)&P05556 (Q21-D728)

Gene ID

3678&3688

Synonyms
Integrin alpha 5 beta 1; VLA-5; ITGA5&ITGB1; Integrin alpha 5 β 1; α5β1
Molecular Weight

110-140 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Integrin alpha 5 beta 1 Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Integrin alpha 5 beta 1 Protein, Human (Biotinylated, HEK293, His-Avi)
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HY-P700755
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