Integrin alpha 5 beta 1 Protein, Human (HEK293, Flag-His)

ITGA5 is a multifunctional receptor that interacts with multiple ligands such as fibronectin, fibrinogen, PLA2G2A, fibrillin-1 (FBN1), IL1B, human metapneumovirus, and soluble CD40LG. ITGA5 recognizes RGD sequences and mediates cell adhesion through different binding sites. Integrin alpha 5 beta 1 Protein, Human (HEK293, Flag-His) is a recombinant protein dimer complex containing human-derived Integrin alpha 5 beta 1 protein, expressed by HEK293 , with C-Flag, C-10*His labeled tag. Integrin alpha 5 beta 1 Protein, Human (HEK293, Flag-His), has molecular weight of ~101.35 kDa.

The Integrin alpha-5/beta-1 protein (ITGA5:ITGB1) functions as a versatile receptor with diverse ligand interactions. Recognizing the R-G-D sequence in its ligands, ITGA5:ITGB1 serves as a receptor for fibronectin and fibrinogen, mediating cell adhesion through distinct binding sites. Notably, it binds to PLA2G2A at a site separate from its classical ligand-binding site, inducing conformational changes that enhance ligand binding. Additionally, ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1), facilitating R-G-D-dependent cell adhesion. It is also a receptor for fibronectin (FN1), enabling R-G-D-dependent cell adhesion to FN1. Furthermore, ITGA5:ITGB1 serves as a receptor for IL1B, playing a crucial role in IL1B signaling. In the context of microbial infection, ITGA5:ITGB1 acts as a receptor for Human metapneumovirus, highlighting its involvement in pathogen recognition. Moreover, ITGA5:ITGB3 acts as a receptor for soluble CD40LG, playing a vital role in CD40/CD40LG signaling. This broad spectrum of ligand interactions underscores the multifunctionality of ITGA5:ITGB1 in cellular processes and signaling pathways.

Immobilized Fibronectin at 2 μg/mL (100 μL/well) can bind Biotinylated Integrin alpha 5 beta 1 protein. The ED₅₀ for this effect is 2.49 ng/mL, corresponding to a specific activity is 4.02×10^5 Unit/mg.

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  Immobilized Fibronectin at 2 μg/mL (100 μL/well) can bind Biotinylated Integrin alpha 5 beta 1 protein. The ED₅₀ for this effect is 2.49 ng/mL, corresponding to a specific activity is 4.02×10⁵ Unit/mg.

Human

HEK293

C-Flag;C-10*His

P08648 (F42-Y995) & P05556-1 (Q21-D728)

3678  [NCBI]&3688  [NCBI]

FNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSY&QTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPD

Approximately 101.35 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.