Intermediate capsid protein VP6, Rotavirus A (sf9, His)

The intermediate capsid protein VP6 self-assembles into an icosahedral capsid with T=13 symmetry, forming the middle layer of the virus particle. It contains 230 VP6 trimers that protect dsRNA and prevent host cell antiviral responses. Intermediate capsid protein VP6, Rotavirus A (sf9, His) is the recombinant Virus-derived Intermediate capsid protein VP6, expressed by Sf9 insect cells , with N-His, C-Myc labeled tag. The total length of Intermediate capsid protein VP6, Rotavirus A (sf9, His) is 397 a.a., with molecular weight of ~45 kDa.

The intermediate capsid protein VP6 functions as a self-assembling component, forming an icosahedral capsid with T=13 symmetry. This capsid, comprising 230 VP6 trimers, constitutes the middle concentric layer of the viral mature particle. During the virus replication cycle, VP6, along with the innermost VP2 capsid, remains intact following cell entry, providing protection to the dsRNA from degradation and preventing unfavorable antiviral responses in the host cell. The VP6 capsid plays a crucial role in the transcription activity of the double-layered particle (DLP), where nascent transcripts are transcribed within its structural confines and extruded through the channels at the five-fold axes. VP6 interacts with the inner capsid protein VP2, the outer capsid glycoprotein VP7, and the outer capsid protein VP5*, forming essential interactions for the overall functionality of the viral capsid.

Virus

Sf9 insect cells

N-6*His

P03530 (M1-K397)

/

MEVLYSLSKTLKDARDKIVEGTLYSNVSDLIQQFNQMIVTMNGNDFQTGGIGNLPVRNWTFDFGLLGTTLLNLDANYVENARTIIEYFIDFIDNVCMDEMARESQRNGVAPQSEALRKLAGIKFKRINFDNSSEYIENWNLQNRRQRTGFVFHKPNIFPYSASFTLNRSQPMHDNLMGTMWLNAGSEIQVAGFDYSCAINAPANIQQFEHIVQLRRALTTATITLLPDAERFSFPRVINSADGATTWFFNPVILRPNNVEVEFLLNGQIINTYQARFGTIIARNFDAIRLLFQLMRPPNMTPAVNALFPQAQPFQHHATVGLTLRIESAVCESVLADANETLLANVTAVRQEYAIPVGPVFPPGMNWTELITNYSPSREDNLQRVFTVASIRSMLIK

Approximately 45 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCL, 500 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.