1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. IVD Protein, Human (sf9, His)

IVD Protein, Human (sf9, His)

Cat. No.: HY-P73857
Handling Instructions

IVD is a key enzyme responsible for catalyzing the conversion of isovaleryl-CoA/3-methylbutyryl-CoA into 3-methylbut-2-enoyl-CoA, which is an important intermediate step in the leucine catabolism pathway. . This enzyme activity is essential for the breakdown of the branched-chain amino acid leucine and contributes to the regulation of metabolic processes. IVD Protein, Human (sf9, His) is the recombinant human-derived IVD protein, expressed by Sf9 insect cells , with N-His labeled tag.

For research use only. We do not sell to patients.

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Description

IVD is a key enzyme responsible for catalyzing the conversion of isovaleryl-CoA/3-methylbutyryl-CoA into 3-methylbut-2-enoyl-CoA, which is an important intermediate step in the leucine catabolism pathway. . This enzyme activity is essential for the breakdown of the branched-chain amino acid leucine and contributes to the regulation of metabolic processes. IVD Protein, Human (sf9, His) is the recombinant human-derived IVD protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

Isovaleryl-CoA dehydrogenase (IVD) is a crucial enzyme in the leucine catabolic pathway, catalyzing the conversion of isovaleryl-CoA/3-methylbutanoyl-CoA to 3-methylbut-2-enoyl-CoA. This enzymatic step is a key intermediate in the breakdown of leucine. In addition to its primary role in leucine metabolism, IVD exhibits the ability to oxidize other saturated short-chain acyl-CoA thioesters, including pentanoyl-CoA, hexenoyl-CoA, and butenoyl-CoA, albeit to a lesser extent. These broader substrate capabilities suggest that IVD may contribute to the oxidation of various short-chain fatty acids, expanding its metabolic role beyond leucine catabolism. The multifunctional nature of IVD underscores its importance in maintaining metabolic homeostasis by participating in the degradation of diverse acyl-CoA substrates (adapted from the provided passage).

Species

Human

Source

Sf9 insect cells

Tag

N-His

Accession

P26440-1 (H33-H426)

Gene ID
Molecular Construction
N-term
His
IVD (H33-H426)
Accession # P26440-1
C-term
Synonyms
Isovaleryl-CoA dehydrogenase, mitochondrial; IVD
Molecular Weight

Approximately 43 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

IVD Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IVD Protein, Human (sf9, His)
Cat. No.:
HY-P73857
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