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  3. Hydrolases (EC 3)
  4. JOSD1 Protein, Human (GST)

JOSD1 Protein, Human (GST)

Cat. No.: HY-P701475
Handling Instructions

The JOSD1 protein is a versatile player in ubiquitin dynamics and exhibits deubiquitinase activity by cleaving monoubiquitinated probes in vitro. When ubiquitinated, JOSD1 can cleave “Lys-63”-linked and “Lys-48”-linked polyubiquitin chains in vitro, suggesting its potential for deubiquitination. JOSD1 Protein, Human (GST) is the recombinant human-derived JOSD1 protein, expressed by E. coli , with N-GST labeled tag. The total length of JOSD1 Protein, Human (GST) is 201 a.a., .

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The JOSD1 protein is a versatile player in ubiquitin dynamics and exhibits deubiquitinase activity by cleaving monoubiquitinated probes in vitro. When ubiquitinated, JOSD1 can cleave “Lys-63”-linked and “Lys-48”-linked polyubiquitin chains in vitro, suggesting its potential for deubiquitination. JOSD1 Protein, Human (GST) is the recombinant human-derived JOSD1 protein, expressed by E. coli , with N-GST labeled tag. The total length of JOSD1 Protein, Human (GST) is 201 a.a., .

Background

JOSD1 Protein, a versatile player in ubiquitin dynamics, exhibits deubiquitinase activity by cleaving monoubiquitinated probes in vitro. Moreover, when ubiquitinated itself, JOSD1 can cleave both 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains in vitro, suggesting a potential role as a deubiquitinating enzyme. Beyond its catalytic functions, JOSD1 is implicated in cellular processes, influencing macropinocytosis positively and concurrently suppressing clathrin- and caveolae-mediated endocytosis. Notably, JOSD1 appears to contribute to membrane dynamics and cell motility independently of its enzymatic activity, highlighting its multifaceted role in orchestrating cellular responses and homeostasis.

Species

Human

Source

E. coli

Tag

N-GST

Accession

Q15040 (S2-V202)

Gene ID

9929

Molecular Construction
N-term
GST
JOSD1 (S2-V202)
Accession # Q15040
C-term
Synonyms
JOSD1; Josephin-1; Josephin domain-containing protein 1
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

JOSD1 Protein, Human (GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
JOSD1 Protein, Human (GST)
Cat. No.:
HY-P701475
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