Kallikrein-11 Protein, Human (HEK293, His)

Kallikrein-11 protein is suggested to be a multifunctional protease, efficiently cleaving the substrate 'bz-Phe-Arg-4-methylcoumaryl-7-amide' and showing weak cleavage activity toward other kallikrein and trypsin substrates. It predominantly cleaves synthetic peptides after arginine residues rather than lysine residues. Kallikrein-11 Protein, Human (HEK293, His) is the recombinant human-derived Kallikrein-11 protein, expressed by HEK293 , with C-His labeled tag.

Kallikrein-11 protein is speculated to be a potential multifunctional protease. It has been shown to efficiently cleave a kallikrein substrate called 'bz-Phe-Arg-4-methylcoumaryl-7-amide' and exhibits weak cleavage activity towards other substrates for kallikrein and trypsin. Notably, it primarily cleaves synthetic peptides after arginine residues rather than lysine residues.

Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al.,1999, J. Biol. Chem. 274: 30468) . The specific activity is >200 pmol/min/μg.
(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Human

HEK293

C-His

Q9UBX7-1 (E19-N250)

11012  [NCBI]

Approximately 40 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.