Kallikrein-11 Protein, Mouse (HEK293, His)

Kallikrein-11 protein, a potential multifunctional protease, demonstrates efficient cleavage of the substrate 'bz-Phe-Arg-4-methylcoumaryl-7-amide,' specific to kallikrein. It also displays weaker cleavage activity toward other substrates linked to kallikrein and trypsin. This multifunctional characteristic indicates versatility in substrate recognition and cleavage, suggesting a potential role in diverse proteolytic processes. Kallikrein-11 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kallikrein-11 protein, expressed by HEK293 , with C-His labeled tag.

Kallikrein-11 protein is proposed as a potential multifunctional protease with demonstrated efficacy in cleaving 'bz-Phe-Arg-4-methylcoumaryl-7-amide,' a substrate specific to kallikrein. Additionally, it exhibits weaker cleavage activity towards other substrates associated with kallikrein and trypsin. This multifunctional nature suggests a versatility in substrate recognition and cleavage, highlighting its potential role in various proteolytic processes.

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is 335.177 pmol/min/μg, as measured under the described conditions.

Mouse

HEK293

C-6*His

Q9QYN3-1 (E45-N276)

56538  [NCBI]

ETRIIKGYECRPHSQPWQVALFQKTRLLCGATLIAPKWLLTAAHCRKPHYVILLGEHNLEKTDGCEQRRMATESFPHPDFNNSLPNKDHRNDIMLVKMSSPVFFTRAVQPLTLSPHCVAAGTSCLISGWGTTSSPQLRLPHSLRCANVSIIEHKECEKAYPGNITDTMLCASVRKEGKDSCQGDSGGPLVCNGSLQGIISWGQDPCAVTRKPGVYTKVCKYFNWIHEVMRNN

Approximately 35-43 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.