Kallikrein-15 Protein, Human (HEK293, C-His)

Kallikrein-15 is a kallikrein (KLK) with trypsin-like activity that may promote cancer metastasis. The study found that Kallikrein-15 is only strongly expressed in mature sperm cells and not in immature germ cells. KLK15 may be able to cleave many extracellular matrix (ECM) components, interact with the extracellular matrix (ECM) receptor group, and be associated with tumorigenesis. Kallikrein-15 Protein, Human (HEK293, His) is the recombinant human-derived Kallikrein-15 protein, expressed by HEK293 , with C-His labeled tag.

Kallikrein-15 (KLK15) is a kallikrein (KLK). Previous research has suggested that KLK15 may play a role in prostate cancer. Chemical analysis of immunohistology of testes showed that KLK15 is strongly expressed in mature sperm cells but not in immature germ cells. The subcellular localization of KLK15 in the basal cell layer of the prostate epithelium is primarily in the nucleus. KLK15 also has trypsin-like activity and preferentially cleaves after arginine (R). KLK15 may be able to cleave many extracellular matrix (ECM) components, functionally similar to some KLK family members. The enzymatic activity of KLK15 is regulated by different factors such as pH, cations, and serine protease inhibitors. Notably, KLK15 is most likely associated with tumorigenesis by interacting with the extracellular matrix (ECM) receptor group and may promote metastasis through this mechanism.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

Q9H2R5-1 (Q17-N256)

55554

QDGDKLLEGDECAPHSQPWQVALYERGRFNCGASLISPHWVLSAAHCQSRFMRVRLGEHNLRKRDGPEQLRTTSRVIPHPRYEARSHRNDIMLLRLVQPARLNPQVRPAVLPTRCPHPGEACVVSGWGLVSHNEPGTAGSPRSQVSLPDTLHCANISIISDTSCDKSYPGRLTNTMVCAGAEGRGAESCEGDSGGPLVCGGILQGIVSWGDVPCDNTTKPGVYTKVCHYLEWIRETMKRN

Approximately 38-45 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.