KARS Protein, Human (HEK293, His)

The KARS protein promotes attachment of amino acids to its cognate tRNA through a two-step reaction, inducing immune responses through monocyte/macrophage activation. In microbial infections, it interacts with the HIV-1 GAG protein to enable selective tRNA(3)(Lys) packaging to initiate reverse transcription. KARS Protein, Human (HEK293, His) is the recombinant human-derived KARS protein, expressed by HEK293 , with C-6*His labeled tag.

KARS protein is responsible for catalyzing the specific attachment of an amino acid to its cognate tRNA through a two-step reaction: the amino acid is initially activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. In addition to its role in protein synthesis, when secreted, KARS acts as a signaling molecule, inducing immune responses by activating monocyte/macrophages. Furthermore, KARS plays a crucial role in synthesizing the signaling molecule diadenosine tetraphosphate (Ap4A), leading to the disruption of the complex between HINT1 and MITF and the subsequent activation of MITF transcriptional activity. In the context of microbial infection, KARS interacts with the HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), which serves as the primer for reverse transcription initiation.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

Q15046-1 (A2-V597)

3735  [NCBI]

AAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

70-90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 20% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.