Kirrel1/NEPH1 Protein, Human (HEK293, His)

The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier in the kidney, maintaining stable podocyte structure through finger-like foot processes and gap septal cell-cell junctions. As a signaling molecule, it requires TEC kinase to fully activate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Human (HEK293, His) is the recombinant human-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-His labeled tag.

Kirrel1/NEPH1 Protein is crucial for the proper function of the glomerular filtration barrier in the kidneys. It plays a vital role in maintaining a stable podocyte architecture by facilitating the interdigitating foot processes connected by specialized cell-cell junctions called the slit diaphragm. This protein acts as a signaling molecule and requires the presence of TEC kinases to fully activate the transcription factor AP-1. Kirrel1/NEPH1 interacts with TJP1/ZO-1 and NPHS2/podocin, and its interaction with NPHS1/nephrin relies on Kirrel1/NEPH1 glycosylation. It forms homodimers through its Ig-like domains and, when tyrosine-phosphorylated, it interacts with GRB2.

Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to KIRREL coated plates (15 μg/mL, 100 μL/well), > 20% will adhere specifically after 90 minutes at 37 °C.

Human

HEK293

C-His

Q96J84-1 (Q17-L493)

55243  [NCBI]

Approximately 53.5 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.