1. Recombinant Proteins
  2. Ubiquitin Related Proteins
  3. KLHL42 Protein, Human (Sf9, His, Strep)

KLHL42 Protein, Human (Sf9, His, Strep)

Cat. No.: HY-P701588
Handling Instructions

KLHL42 serves as a substrate-specific linker in the BCR E3 ubiquitin ligase complex and plays a critical role in mitotic progression and cytokinesis. The BCR(KLHL42) complex actively ubiquitinates and degrades KATNA1, contributing to the regulation of microtubule dynamics during mitosis. KLHL42 Protein, Human (Sf9, His, Strep) is the recombinant human-derived KLHL42 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of KLHL42 Protein, Human (Sf9, His, Strep) is 504 a.a., .

For research use only. We do not sell to patients.

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Description

KLHL42 serves as a substrate-specific linker in the BCR E3 ubiquitin ligase complex and plays a critical role in mitotic progression and cytokinesis. The BCR(KLHL42) complex actively ubiquitinates and degrades KATNA1, contributing to the regulation of microtubule dynamics during mitosis. KLHL42 Protein, Human (Sf9, His, Strep) is the recombinant human-derived KLHL42 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of KLHL42 Protein, Human (Sf9, His, Strep) is 504 a.a., .

Background

KLHL42, functioning as the substrate-specific adapter within the BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex, assumes a pivotal role in mitotic progression and cytokinesis. The BCR(KLHL42) E3 ubiquitin ligase complex orchestrates these essential cellular processes by actively mediating the ubiquitination and subsequent degradation of KATNA1, thereby contributing to the regulation of microtubule dynamics throughout mitosis. The involvement of KLHL42 in protein modification, specifically protein ubiquitination, highlights its significance in fine-tuning the cellular events crucial for proper mitotic progression and cytokinesis. The intricate interplay within the BCR(KLHL42) complex underscores its importance in maintaining the delicate balance required for the dynamic processes associated with cell division.

Species

Human

Source

Sf9 insect cells

Tag

N-Strep;N-8*His

Accession

Q9P2K6 (S2-T505)

Gene ID

57542

Molecular Construction
N-term
8*His-Strep
KLHL42 (S2-T505)
Accession # Q9P2K6
C-term
Synonyms
KLHL42; Kelch-like protein 42; Cullin-3-binding protein 9; Ctb9; Kelch domain-containing protein 5
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

KLHL42 Protein, Human (Sf9, His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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KLHL42 Protein, Human (Sf9, His, Strep)
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HY-P701588
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