Klotho Protein, Human (CHO, His)

Klotho Protein exhibits weak glycosidase activity against glucuronidated steroids and is inactive in vivo due to the lack of essential active site Glu residues. It regulates calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Klotho Protein, Human (CHO, His) is the recombinant human-derived Klotho protein, expressed by CHO , with C-10*His labeled tag. The total length of Klotho Protein, Human (CHO, His) is 948 a.a., with molecular weight of 120-140 kDa.

The Klotho protein exhibits weak glycosidase activity towards glucuronylated steroids, but its in vivo inactivity as a glycosidase is suggested by the absence of essential active site Glu residues at positions 239 and 872. Additionally, Klotho may play a role in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. As an essential factor for the specific interaction between FGF23 and FGFR1, Klotho contributes to crucial signaling pathways. Furthermore, the Klotho peptide, generated by cleaving the membrane-bound isoform, is proposed to function as an anti-aging circulating hormone. This hormone has the potential to extend lifespan by inhibiting insulin/IGF1 signaling, presenting Klotho as a multifunctional protein with implications in both metabolic regulation and the aging process.

The EC₅₀ as determined by the dose-dependent stimulation of the proliferation of murine NIH/3T3 is less than 1 μg/mL.

Human

CHO

C-10*His

Q9UEF7 (E34-S981)

9365  [NCBI]

EPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDAYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGIRGSPRLGYLVAHNLLLAHAKVWHLYNTSFRPTQGGQVSIALSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPVFIDGDYPESMKNNLSSILPDFTESEKKFIKGTADFFALCFGPTLSFQLLDPHMKFRQLESPNLRQLLSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFYQKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVDTTLSQFTDLNVYLWDVHHSKRLIKVDGVVTKKRKSYCVDFAAIQPQIALLQEMHVTHFRFSLDWALILPLGNQSQVNHTILQYYRCMASELVRVNITPVVALWQPMAPNQGLPRLLARQGAWENPYTALAFAEYARLCFQELGHHVKLWITMNEPYTRNMTYSAGHNLLKAHALAWHVYNEKFRHAQNGKISIALQADWIEPACPFSQKDKEVAERVLEFDIGWLAEPIFGSGDYPWVMRDWLNQRNNFLLPYFTEDEKKLIQGTFDFLALSHYTTILVDSEKEDPIKYNDYLEVQEMTDITWLNSPSQVAVVPWGLRKVLNWLKFKYGDLPMYIISNGIDDGLHAEDDQLRVYYMQNYINEALKAHILDGINLCGYFAYSFNDRTAPRFGLYRYAADQFEPKASMKHYRKIIDSNGFPGPETLERFCPEEFTVCTECSFFHTRKS

120-140kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, 50% Glycerol, pH7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH_2O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.