KREMEN1 Protein, Human (HEK293, His)

KREMEN1 Protein, Human (HEK293, His) is the recombinant human-derived KREMEN1 protein, expressed by HEK293 , with C-His labeled tag. The total length of KREMEN1 Protein, Human (HEK293, His) is 375 a.a., with molecular weight of ~60 KDa.

Measured by its binding ability in a functional ELISA. Immobilized human Dkk-1 at 2 μg/mL can bind Human KREMEN1. The ED₅₀ for this effect is 0.315 μg/mL.

Human

HEK293

C-His

Q96MU8-2/NP_114434.3 (A20-T394)

83999  [NCBI]

ARPAPSPGLGPGPECFTANGADYRGTQNWTALQGGKPCLFWNETFQHPYNTLKYPNGEGGLGEHNYCRNPDGDVSPWCYVAEHEDGVYWKYCEIPACQMPGNLGCYKDHGNPPPLTGTSKTSNKLTIQTCISFCRSQRFKFAGMESGYACFCGNNPDYWKYGEAASTECNSVCFGDHTQPCGGDGRIILFDTLVGACGGNYSAMSSVVYSPDFPDTYATGRVCYWTIRVPGASHIHFSFPLFDIRDSADMVELLDGYTHRVLARFHGRSRPPLSFNVSLDFVILYFFSDRINQAQGFAVLYQAVKEELPQERPAVNQTVAEVITEQANLSVSAARSSKVLYVITTSPSHPPQTVPGSNSWAPPMGAGSHRVEGWT

Approximately 60 kDa. The protein migrates as an approximately 60 kDa band under reducing SDS-PAGE due to glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.