Large envelope Protein, HBV-D (Cell-Free, His)

The large envelope protein has two conformations: “external” (Le-HBsAg) and “internal” (Li-HBsAg). In its external state, it mediates viral attachment, initiates infection, determines species specificity, and promotes viral particle internalization via caveolin-mediated endocytosis. Large envelope Protein, HBV-D (Cell-Free, His) is the recombinant Virus-derived Large envelope protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Large envelope Protein, HBV-D (Cell-Free, His) is 388 a.a., with molecular weight of Monomer:40 kDa Dimer:80 kDa.

The Large Envelope Protein exhibits two distinct topological conformations, known as 'external' or Le-HBsAg and 'internal' or Li-HBsAg. In its external conformation, the protein functions as a key mediator, attaching the virus to cell receptors and initiating infection. This interaction dictates species specificity and liver tropism, prompting virion internalization primarily through caveolin-mediated endocytosis. Additionally, the Large Envelope Protein facilitates fusion between the virion membrane and the endosomal membrane. In its internal conformation, the protein plays a vital role in virion morphogenesis, functioning akin to a matrix protein and mediating contact with the nucleocapsid. Concurrently, the middle envelope protein assumes a crucial role in virion budding, inducing a nucleocapsid-independent process. This budding process results in the majority of envelope proteins forming subviral lipoprotein particles with a diameter of 22 nm, devoid of a nucleocapsid.

Virus

E. coli Cell-free

N-10*His

P03138 (G2-I389)

/

GQNLSTSNPLGFFPDHQLDPAFRANTANPDWDFNPNKDTWPDANKVGAGAFGLGFTPPHGGLLGWSPQAQGILQTLPANPPPASTNRQSGRQPTPLSPPLRNTHPQAMQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSIFSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCLGQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCPLIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWASARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI

Monomer:40 kDa Dimer:80 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.