Latent TGF beta 1/TGFB1 Protein, Cynomolgus (C33S, HEK293, His)

Latent TGF beta 1/TGFB1 Protein is a large extracellular matrix protein and an associated ligand of fibrillinmicrofibrils. Latent TGF beta 1/TGFB1 Protein, Cynomolgus (C33S, HEK293, His) is a recombinant protein (L30-S390, C33S) produced by HEK293 cells with His tag^[1][2].

Latent TGF beta 1/TGFB1 Protein promotes fibrillin-1 and -2 assembly downstream of fibronectin in mouse embryonic fibroblasts^[3].

LTBP-1 fragments (30 µg/mL) inhibit the incorporation of native LTBP-1 into fibroblast matrix^[2].

Measured by its ability to inhibit proliferation of HT-2 mouse T cells. The ED₅₀ this effect is 1.306 ng/ml, corresponding to a specific activity is 7.66×105 units/mg.

• 
  Measured by its ability to inhibit proliferation of HT-2 mouse T cells. The ED₅₀ for this effect is 0.1047ng/mL, corresponding to a specific activity is 9.551×10⁶ units/mg.

Cynomolgus

HEK293

N-His

XP_005589396.1 (L30-S390, C33S)

102129546  [NCBI]

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSKDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

approximately 50-60, 35-45, 13 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. J Taipale, et al. Latent transforming growth factor-beta 1 associates to fibroblast extracellular matrix via latent TGF-beta binding protein. J Cell Biol. 1994 Jan;124(1-2):171-81.  [Content Brief]

  [2]. C Unsöld, et al. Latent TGF-beta binding protein LTBP-1 contains three potential extracellular matrix interacting domains. J Cell Sci. 2001 Jan;114(Pt 1):187-197.  [Content Brief]

  [3]. Matthias Przyklenk, et al. LTBP1 promotes fibrillin incorporation into the extracellular matrix. bioRxiv. 2022.

  [4]. Rui Cai, et al. LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation. J Transl Med. 2020 Mar 26;18(1):139.  [Content Brief]

  [5]. Xiaojun Fu, et al. LTBP1 plays a potential bridge between depressive disorder and glioblastoma. J Transl Med. 2020 Oct 15;18(1):391.  [Content Brief]