Leukotriene A4 Hydrolase/LTA4H Protein, Mouse (sf9, His)

The LTA4H protein is a bifunctional zinc metalloenzyme that acts as an epoxide hydrolase to convert leukotriene A4 (LTA4) into proinflammatory leukotriene B4 (LTB4).It also acts as an aminopeptidase, cleaving N-terminal arginine and fighting inflammation, particularly through Pro-Gly-Pro (PGP) cleavage.Leukotriene A4 Hydrolase/LTA4H Protein, Mouse (sf9, His) is the recombinant mouse-derived Leukotriene A4 Hydrolase/LTA4H protein, expressed by Sf9 insect cells , with C-His labeled tag.

The Leukotriene A4 Hydrolase/LTA4H protein is a bifunctional zinc metalloenzyme with both epoxide hydrolase (EH) and aminopeptidase activities. Serving as an epoxide hydrolase, it catalyzes the conversion of leukotriene A4 (LTA4) to the pro-inflammatory mediator leukotriene B4 (LTB4). Additionally, LTA4H exhibits aminopeptidase activity, displaying a high affinity for N-terminal arginines in various synthetic tripeptides. Beyond its pro-inflammatory EH role, the protein may counteract inflammation through its aminopeptidase activity, which cleaves the neutrophil attractant tripeptide Pro-Gly-Pro (PGP)—a bioactive collagen fragment generated by matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL). Moreover, LTA4H participates in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, contributing to the generation of lipid mediators with potent anti-inflammatory and pro-resolving actions. The multifaceted activities of LTA4H highlight its intricate involvement in the regulation of inflammatory processes and lipid mediator biosynthesis.

Mouse

Sf9 insect cells

C-His

P24527 (M1-D611)

16993  [NCBI]

Approximately 62 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.