1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. LP Protein, Human

LP Protein, Human

Cat. No.: HY-P701477
COA Handling Instructions

AMSHLP is a zinc metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains and acts as a positive regulator of the TORC1 signaling pathway. It specifically deubiquitinates SESN2, disrupts its interaction with the GATOR2 complex and inhibits SESN2-mediated regulation of TORC1. LP Protein, Human is the recombinant human-derived LP protein, expressed by E. coli , with tag free. The total length of LP Protein, Human is 435 a.a., .

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $250 In-stock
50 μg $685 In-stock
100 μg $1160 Get quote
> 100 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

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Description

AMSHLP is a zinc metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains and acts as a positive regulator of the TORC1 signaling pathway. It specifically deubiquitinates SESN2, disrupts its interaction with the GATOR2 complex and inhibits SESN2-mediated regulation of TORC1. LP Protein, Human is the recombinant human-derived LP protein, expressed by E. coli , with tag free. The total length of LP Protein, Human is 435 a.a., .

Background

AMSHLP, a zinc metalloprotease, exhibits a selective ability to cleave 'Lys-63'-linked polyubiquitin chains, without affecting 'Lys-48'-linked polyubiquitin chains. Notably, AMSHLP serves as a positive regulator of the TORC1 signaling pathway through its role in the 'Lys-63'-linked deubiquitination of SESN2. This deubiquitination activity impedes the interaction between SESN2 and the GATOR2 complex, thereby contributing to the inhibition of SESN2-mediated TORC1 regulation. These functions underscore AMSHLP's specific involvement in fine-tuning cellular signaling pathways, particularly those associated with TORC1, through precise modulation of 'Lys-63'-linked polyubiquitin chains.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q96FJ0 (D2-R436)

Gene ID

57559

Molecular Construction
N-term
LP (D2-R436)
Accession # Q96FJ0
C-term
Synonyms
STAMBPL1; AMSH-like protease; AMSH-LP; STAM-binding protein-like 1
Molecular Weight

49.8 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 10 mM HEPES, 150 mM NaCl, 5% glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

LP Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
LP Protein, Human
Cat. No.:
HY-P701477
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