LP Protein, Human

AMSHLP is a zinc metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains and acts as a positive regulator of the TORC1 signaling pathway. It specifically deubiquitinates SESN2, disrupts its interaction with the GATOR2 complex and inhibits SESN2-mediated regulation of TORC1. LP Protein, Human is the recombinant human-derived LP protein, expressed by E. coli , with tag free. The total length of LP Protein, Human is 435 a.a., .

AMSHLP, a zinc metalloprotease, exhibits a selective ability to cleave 'Lys-63'-linked polyubiquitin chains, without affecting 'Lys-48'-linked polyubiquitin chains. Notably, AMSHLP serves as a positive regulator of the TORC1 signaling pathway through its role in the 'Lys-63'-linked deubiquitination of SESN2. This deubiquitination activity impedes the interaction between SESN2 and the GATOR2 complex, thereby contributing to the inhibition of SESN2-mediated TORC1 regulation. These functions underscore AMSHLP's specific involvement in fine-tuning cellular signaling pathways, particularly those associated with TORC1, through precise modulation of 'Lys-63'-linked polyubiquitin chains.

Human

E. coli

Tag Free

Q96FJ0 (D2-R436)

57559

49.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 10 mM HEPES, 150 mM NaCl, 5% glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.