LpxC Protein, E.coli (His)

LpxC protein serves as a key enzyme in lipid A biosynthesis by catalyzing the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine, marking a key and crucial step in this important pathway. Through this enzymatic process, LpxC promotes the conversion of its substrate into UDP-3-O-myristoylglucosamine and acetate, making a significant contribution to lipid A biosynthesis. LpxC Protein, E.coli (His) is the recombinant E. coli-derived LpxC protein, expressed by E. coli , with N-His labeled tag.

LpxC is an enzyme that plays a crucial role in lipid A biosynthesis by catalyzing the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine. This reaction results in the formation of UDP-3-O-myristoylglucosamine and acetate, representing the committed step in the biosynthesis of lipid A, a key component of bacterial lipopolysaccharides. Lipid A is an essential structural element in the outer membrane of Gram-negative bacteria, contributing to membrane integrity and playing a role in host-pathogen interactions. LpxC's catalytic activity is pivotal for the production of lipid A, and the enzyme serves as a potential target for the development of antibacterial agents aimed at disrupting the synthesis of crucial bacterial cell wall components.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

N-His

P0A725 (M1-A305)

944816  [NCBI]

MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDLNPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIPIMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTIDFNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVVDDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVLAKQEAWEYVTFQDDAELPLAFKAPSAVLA

Approximately 38.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.