1. Recombinant Proteins
  2. Receptor Proteins
  3. Nuclear Receptor Superfamily
  4. LXR-α Protein, Human (His)

LXR-α Protein, Human (His)

Cat. No.: HY-P701405
Handling Instructions

LXR-α protein is a nuclear receptor that activates transcription by interacting with RXR. It regulates cholesterol uptake through MYLIP-dependent ubiquitination and is critical for cholesterol homeostasis. LXR-α Protein, Human (His) is the recombinant human-derived LXR-α protein, expressed by E. coli , with N-6*His labeled tag. The total length of LXR-α Protein, Human (His) is 266 a.a., .

For research use only. We do not sell to patients.

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Description

LXR-α protein is a nuclear receptor that activates transcription by interacting with RXR. It regulates cholesterol uptake through MYLIP-dependent ubiquitination and is critical for cholesterol homeostasis. LXR-α Protein, Human (His) is the recombinant human-derived LXR-α protein, expressed by E. coli , with N-6*His labeled tag. The total length of LXR-α Protein, Human (His) is 266 a.a., .

Background

LXR-α Protein, a nuclear receptor, displays ligand-dependent transcriptional activation activity, and its interaction with retinoic acid receptor (RXR) transforms RXR into an active ligand-binding subunit, mediating retinoid responses through target genes defined by LXRES. These LXRES consist of DR4-type response elements characterized by direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides. A pivotal player in cholesterol homeostasis, LXR-α regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8. Functionally interacting with RORA, it contributes to the regulation of genes involved in liver metabolism. Moreover, LXR-α induces LPCAT3-dependent phospholipid remodeling in hepatocyte endoplasmic reticulum membranes, facilitating SREBF1 processing and lipogenesis. Through LPCAT3, it promotes the incorporation of arachidonate into phosphatidylcholines, enhancing membrane dynamics and enabling the transfer of triacylglycerols to nascent very low-density lipoprotein particles. Additionally, LXR-α, via LPCAT3, counteracts lipid-induced ER stress response and inflammation by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators. Forming a heterodimer with NR1H3 and RXR, LXR-α also interacts with CCAR2, SIRT1, and GPS2, further illustrating its intricate regulatory network.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

Q13133 (Q182-E447)

Gene ID

10062

Molecular Construction
N-term
6*His
LXR-α (Q182-E447)
Accession # Q13133
C-term
Synonyms
NR1H3; Oxysterols receptor LXR-alpha; Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

LXR-α Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LXR-α Protein, Human (His)
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