MAdCAM1 Protein, Mouse (HEK293, His)

MAdCAM1, expressed in mucosal venules, acts as a crucial cell adhesion leukocyte receptor, directing lymphocyte traffic into mucosal tissues. It regulates leukocyte passage and retention, effectively binding to integrin alpha-4/beta-7 and L-selectin for comprehensive leukocyte adhesion control. Both isoforms 1 and 2 adhere to integrin alpha-4/beta-7, with isoform 2, lacking the mucin-like domain, potentially specializing in integrin-dependent adhesion strengthening, independently of L-selectin binding. MAdCAM1 forms homodimers to mediate cell adhesion. MAdCAM1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MAdCAM1 protein, expressed by HEK293, with C-His labeled tag. The total length of MAdCAM1 Protein, Mouse (HEK293, His) is 343 a.a., with molecular weight of 60-70 kDa.

MAdCAM1 protein, expressed by mucosal venules, serves as a cell adhesion leukocyte receptor crucial for directing lymphocyte traffic into mucosal tissues, including the Peyer patches and the intestinal lamina propria. This protein plays a pivotal role in regulating both the passage and retention of leukocytes. MAdCAM1 can effectively bind to both integrin alpha-4/beta-7 and L-selectin, facilitating comprehensive control over leukocyte adhesion. Both isoform 1 and isoform 2 of MAdCAM1 demonstrate the ability to adhere to integrin alpha-4/beta-7. Notably, isoform 2, distinguished by its lack of the mucin-like domain, may specialize in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independently of L-selectin binding. The protein forms homodimers to accomplish its functional roles in mediating cell adhesion.

Immobilized Human FcRH5, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Ant-FcRH5 Antibody, hFc Tag with the EC₅₀ of 12.5 ng/mL determined by ELISA.

Mouse

HEK293

C-His

Q61826-1 (Q22-S364)

/

QSFQVNPPESEVAVAMGTSLQITCSMSCDEGVARVHWRGLDTSLGSVQTLPGSSILSVRGMLSDTGTPVCVGSCGSRSFQHSVKILVYAFPDQLVVSPEFLVPGQDQVVSCTAHNIWPADPNSLSFALLLGEQRLEGAQALEPEQEEEIQEAEGTPLFRMTQRWRLPSLGTPAPPALHCQVTMQLPKLVLTHRKEIPVLQSQTSPKPPNTTSAEPYILTSSSTAEAVSTGLNITTLPSAPPYPKLSPRTLSSEGPCRPKIHQDLEAGWELLCEASCGPGVTVRWTLAPGDLATYHKREAGAQAWLSVLPPGPMVEGWFQCRQDPGGEVTNLYVPGQVTPNSSS

60-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.