MAG/Siglec-4a Protein, Rat (HEK293, His)

MAG/Siglec-4a protein binds gangliosides, RTN4R, and RTN4RL2, mediating myelinating cell-neuron interactions. It maintains axon myelination, protects motoneurons, prevents axon degeneration, and inhibits neurite outgrowth. It exists as a monomer or homodimer, interacting with BSG isoform 2. MAG/Siglec-4a Protein, Rat (HEK293, His) is the recombinant rat-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-10*His labeled tag. The total length of MAG/Siglec-4a Protein, Rat (HEK293, His) is 497 a.a., .

The MAG/Siglec-4a protein is an adhesion molecule that plays a crucial role in mediating interactions between myelinating cells and neurons. It accomplishes this by binding to neuronal sialic acid-containing gangliosides, as well as the glycoproteins RTN4R and RTN4RL2. While it is not necessary for initial myelination, it appears to be involved in maintaining normal axon myelination and protecting motoneurons from apoptosis, even after injury. The protein is also required to prevent degeneration of myelinated axons in adults, likely through its binding to gangliosides on the axon cell membrane. Additionally, it acts as a negative regulator of neurite outgrowth, inhibiting both axon longitudinal growth and axon outgrowth by binding to RTN4R. Its inhibitory effects on neurite extension are primarily mediated by its interaction with neuronal RTN4R, RTN4RL2, and gangliosides. The MAG/Siglec-4a protein can exist as a monomer or homodimer and interacts with isoform 2 of BSG through its first three N-terminal Ig-like domains.

Measured by its ability to inhibit proliferation of SH-SY5Y cells. The ED₅₀ for this effect is 2.501 μg/mL.

Rat

HEK293

C-10*His

P07722 (G20-P516)

29409

GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIINTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAESLYLDLEEVTPAEDGIYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTLRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP

Approximately 75-95 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.