MAP4K2 Protein, Human (sf9, His-GST)

MAP4K2 is a serine/threonine protein kinase and an important component of the MAP kinase signal transduction pathway. It acts as a MAPK kinase kinase (MAP4K), activating the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) pathway and, to a lesser extent, the p38 MAPKs pathway. MAP4K2 Protein, Human (sf9, His-GST) is the recombinant human-derived MAP4K2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of MAP4K2 Protein, Human (sf9, His-GST) is 812 a.a., with molecular weight of ~116 kDa.

MAP4K2, a serine/threonine-protein kinase, serves as an integral component in the MAP kinase signal transduction pathway, functioning as a MAPK kinase kinase kinase (MAP4K). It acts as a crucial upstream activator primarily for the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway, and to a lesser extent, for the p38 MAPKs signaling pathway. MAP4K2 is essential for the efficient activation of JNKs in response to TRAF6-dependent stimuli, including various pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), and bacterial flagellin. Additionally, it contributes to JNKs activation in response to IL-1 and engagement of CD40. Notably, MAP4K2 plays a prominent role in LPS signaling, impacting c-Jun phosphorylation and IL-8 induction. Furthermore, it enhances the oligomerization of MAP3K1, potentially relieving N-terminal mediated MAP3K1 autoinhibition and facilitating activation through autophosphorylation. MAP4K2 also mediates the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway by activating the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. Its involvement may extend to the regulation of vesicle targeting or fusion.

Human

Sf9 insect cells

N-His;N-GST

Q12851-2 (M1-Y812)

5871  [NCBI]

Approximately 116 kDa

Greater than 87% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.