Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His)

Mast Cell Protease-1 (MCPT-1) displays chymotrypsin-like enzymatic activity.Mast Cell Protease-1/MCPT-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Mast Cell Protease-1/MCPT-1 protein, expressed by HEK293 , with C-His labeled tag.

Mast Cell Protease-1 (MCPT-1) exhibits a chymotrypsin-like enzymatic activity.

Measured by its ability to cleave Suc-Ala-Ala-Pro-Phe-AMC. The specific activity is 51.502 pmol/min/μg, as measured under the described conditions.

Mouse

HEK293

C-6*His

P11034 (E19-K246)

17224  [NCBI]

EEIIGGVEARPHSRPYMAHLKIITDRGSEDRCGGFLIAPQFVLTAAHCKGREITVTLGAHDVSKSESTQQRIKVEKQIIHKNYNVSFNLYDIMLLKLEEKAELTPTVDVIPLPGPSDFIDPGKMCWTAGWGKTGEKEPTSETLREVELRIMDKEACKMYKHYDYNFQVCVGSSTKLKTAYMGDSGGPLLCAGVAHGIVSYGDSHGKPPAVFTRISAYVPWIKTVINGK

Approximately 30-32 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.