MCAD Protein, Human (His)

MCAD proteins play a key role in mitochondrial fatty acid beta-oxidation, initiating the breakdown of fatty acids into acetyl-CoA for energy production. Specifically, MCAD catalyzes the removal of hydrogen from C-2 and C-3 of linear fatty acyl-CoA thioesters to form trans-2-enoyl-CoA. MCAD Protein, Human (His) is the recombinant human-derived MCAD protein, expressed by E. coli , with N-His labeled tag. The total length of MCAD Protein, Human (His) is 396 a.a., with molecular weight of ~42.0 kDa.

MCAD Protein serves a pivotal role as one of the acyl-CoA dehydrogenases involved in the initial stage of mitochondrial fatty acid beta-oxidation, a vital aerobic process that breaks down fatty acids into acetyl-CoA, thus enabling energy production from fats. In this process, MCAD catalyzes the removal of a hydrogen from C-2 and C-3 of straight-chain fatty acyl-CoA thioesters, leading to the formation of trans-2-enoyl-CoA. The subsequent transfer of electrons to the main mitochondrial respiratory chain is facilitated by the electron acceptor Electron Transfer Flavoprotein (ETF), which, in turn, conveys electrons to ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Notably, MCAD exhibits specificity for acyl-CoAs with saturated primary chains ranging from 6 to 12 carbons in length. The intricate orchestration of these molecular events underscores MCAD's indispensable role in the metabolic pathway, contributing to the efficient utilization of fatty acids as an energy source in mitochondria.

Human

E. coli

N-His

P11310 (K26-N421)

34  [NCBI]

HHHHHHKANRQREPGLGFSFEFTEQQKEFQATARKFAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTSQIQRLIVAREHIDKYKN

Approximately 42.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filter solution of 20 mM Tris, 100 mM NaCl, 20% Glycerol, pH 8.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. K Tanaka,et al. Mutations in the Medium Chain acyl-CoA Dehydrogenase (MCAD) Gene. Hum Mutat. 1992;1(4):271-9.  [Content Brief]