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MCAD Protein, Human (His)

Cat. No.: HY-P7451
COA Handling Instructions

MCAD proteins play a key role in mitochondrial fatty acid beta-oxidation, initiating the breakdown of fatty acids into acetyl-CoA for energy production. Specifically, MCAD catalyzes the removal of hydrogen from C-2 and C-3 of linear fatty acyl-CoA thioesters to form trans-2-enoyl-CoA. MCAD Protein, Human (His) is the recombinant human-derived MCAD protein, expressed by E. coli , with N-His labeled tag. The total length of MCAD Protein, Human (His) is 396 a.a., with molecular weight of ~42.0 kDa.

For research use only. We do not sell to patients.

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Description

MCAD proteins play a key role in mitochondrial fatty acid beta-oxidation, initiating the breakdown of fatty acids into acetyl-CoA for energy production. Specifically, MCAD catalyzes the removal of hydrogen from C-2 and C-3 of linear fatty acyl-CoA thioesters to form trans-2-enoyl-CoA. MCAD Protein, Human (His) is the recombinant human-derived MCAD protein, expressed by E. coli , with N-His labeled tag. The total length of MCAD Protein, Human (His) is 396 a.a., with molecular weight of ~42.0 kDa.

Background

MCAD Protein serves a pivotal role as one of the acyl-CoA dehydrogenases involved in the initial stage of mitochondrial fatty acid beta-oxidation, a vital aerobic process that breaks down fatty acids into acetyl-CoA, thus enabling energy production from fats. In this process, MCAD catalyzes the removal of a hydrogen from C-2 and C-3 of straight-chain fatty acyl-CoA thioesters, leading to the formation of trans-2-enoyl-CoA. The subsequent transfer of electrons to the main mitochondrial respiratory chain is facilitated by the electron acceptor Electron Transfer Flavoprotein (ETF), which, in turn, conveys electrons to ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Notably, MCAD exhibits specificity for acyl-CoAs with saturated primary chains ranging from 6 to 12 carbons in length. The intricate orchestration of these molecular events underscores MCAD's indispensable role in the metabolic pathway, contributing to the efficient utilization of fatty acids as an energy source in mitochondria.

Species

Human

Source

E. coli

Tag

N-His

Accession

P11310 (K26-N421)

Gene ID

34  [NCBI]

Molecular Construction
N-term
His
MCAD (K26-N421)
Accession # P11310
C-term
Synonyms
rHuMCAD, His; ACADM; MCAD
AA Sequence

HHHHHHKANRQREPGLGFSFEFTEQQKEFQATARKFAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTSQIQRLIVAREHIDKYKN

Molecular Weight

Approximately 42.0 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filter solution of 20 mM Tris, 100 mM NaCl, 20% Glycerol, pH 8.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

MCAD Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MCAD Protein, Human (His)
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