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  4. MCD Protein, Human (His)

MCD Protein, Human (His)

Cat. No.: HY-P701982
COA Handling Instructions

MCD proteins catalyze the conversion of malonyl-CoA to acetyl-CoA, ensuring that only methylmalonyl-CoA serves as a substrate for fatty acid synthase, producing fatty acids with multiple methyl side chains. MCD Protein, Human (His) is the recombinant human-derived MCD protein, expressed by E. coli , with N-6*His labeled tag. The total length of MCD Protein, Human (His) is 452 a.a., .

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $165 In-stock
50 μg $425 In-stock
100 μg $680 Get quote
> 100 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

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Description

MCD proteins catalyze the conversion of malonyl-CoA to acetyl-CoA, ensuring that only methylmalonyl-CoA serves as a substrate for fatty acid synthase, producing fatty acids with multiple methyl side chains. MCD Protein, Human (His) is the recombinant human-derived MCD protein, expressed by E. coli , with N-6*His labeled tag. The total length of MCD Protein, Human (His) is 452 a.a., .

Background

The MCD protein plays a pivotal role in cellular metabolism by catalyzing the conversion of malonyl-CoA to acetyl-CoA. Particularly significant in fatty acid biosynthesis, MCD selectively eliminates malonyl-CoA, ensuring that methyl-malonyl-CoA becomes the exclusive substrate for fatty acid synthase. This selective removal mechanism contributes to the production of fatty acids with multiple methyl side chains. In peroxisomes, MCD is implicated in the degradation of intraperoxisomal malonyl-CoA, a byproduct of peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Beyond its involvement in lipid metabolism, MCD influences the metabolic balance between glucose and lipid oxidation in muscle, independent of insulin signaling alterations. Additionally, MCD may play a role in mitigating ischemic injury by promoting glucose oxidation, highlighting its multifaceted impact on cellular functions and metabolic pathways.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

O95822 (M40-S491)(E58A, K59A, E278A, E279A, K280A)

Gene ID

23417

Molecular Construction
N-term
6*His
MCD (M40-S491)
Accession # O95822
C-term
Synonyms
MLYCD; Malonyl-CoA decarboxylase; mitochondrial; MCD
Molecular Weight

Approximately 52.4 KDa.

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

MCD Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
MCD Protein, Human (His)
Cat. No.:
HY-P701982
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