MDH2 Protein, Human (HEK293, His)

MDH2 is a key enzyme that undergoes acetylation, leading to an increase in its enzymatic activity. This post-translational modification acts as a regulatory mechanism for MDH2, potentially affecting its function and playing a role in the regulation of cellular metabolic processes. MDH2 Protein, Human (HEK293, His) is the recombinant human-derived MDH2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MDH2 Protein, Human (HEK293, His) is 314 a.a., with molecular weight of ~34.0 kDa.

MDH2, a critical enzyme, undergoes acetylation, which leads to an increase in its enzymatic activity. This post-translational modification acts as a regulatory mechanism for MDH2, potentially impacting its functionality and playing a role in the modulation of cellular metabolic processes. Understanding the specific effects of acetylation on MDH2 can provide insights into the regulation of metabolic pathways and may have implications for diseases related to metabolic dysregulation.

The specific activity is 58.31U/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25°C.

Human

HEK293

C-6*His

P40926 (A25-K338)

4191  [NCBI]

AKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK

Approximately 34.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.