MERS-CoV Spike/S1 Protein (CHO, hFc)

MERS-CoV Spike/S1 Proteinas, a crucial component of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV), mediates viral entry into host cells by binding to the host receptor DPP4. The Spike/S1 protein initiates the infection process and is a key target for antiviral strategies. Understanding its structure and interactions is vital for developing effective interventions against MERS-CoV. MERS-CoV Spike/S1 Protein (CHO, hFc) is the recombinant Virus-derived MERS-CoV Spike/S1 protein, expressed by CHO , with C-hFc labeled tag.

ABCD2, an ATP-dependent transporter belonging to the ATP-binding cassette (ABC) family, plays a crucial role in facilitating the transport of very long-chain fatty acid (VLCFA)-CoA from the cytosol to the peroxisome lumen. Similar to ABCD1, ABCD2 exhibits fatty acyl-CoA thioesterase (ACOT) and ATPase activities. According to the proposed model, VLCFA-CoA is transported in an ATP-dependent manner into peroxisomes after the hydrolysis of VLCFA-CoA, mediated by ABCD2's ACOT activity (probable). Notably, ABCD2 shares overlapping substrate specificities with ABCD1, particularly towards saturated fatty acids (FA) and monounsaturated FA (MUFA). However, ABCD2 demonstrates a distinct substrate preference for shorter VLCFA (C22:0) and polyunsaturated fatty acids (PUFA) such as C22:6-CoA and C24:6-CoA, as observed in vitro. This substrate specificity suggests a potential regulatory role for ABCD2 in very long-chain fatty acids (VLCFAs) and energy metabolism, particularly in the degradation and biosynthesis of fatty acids through beta-oxidation.

Measured by its binding ability in a functional ELISA. When Recombinant Human DPPIV/CD26 (HY-P72975) is present at 5 μg/mL can bind Recombinant MERS-CoV Spike/S1. The ED₅₀ for this effect is 0.4222 μg/mL.

Virus

CHO

C-hFc

K9N5Q8 (Y18-P747)

1489668

YVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQGRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSIRSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGVYSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRFLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVSVIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNSSLFVEDCKLPLGQSLCALPDTPSTLTP

110-135 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.