MFN2 Protein, Human (Cell-Free, His)

MFN2, a mitochondrial outer membrane GTPase, regulates mitochondrial dynamics, balancing fusion and fission for dynamic organelle morphology. Its overexpression forms extensive mitochondrial networks, highlighting its organizational role. Beyond shaping structure, MFN2 influences mitochondrial metabolism, impacting obesity and apoptosis. In vascular smooth muscle cells, it regulates proliferation. Crucial in mitochondrial quality control, MFN2 facilitates mitophagy and modulates the unfolded protein response during ER stress, emphasizing its diverse roles in cellular homeostasis and stress responses. MFN2 Protein, Human (Cell-Free, His) is the recombinant human-derived MFN2 protein, expressed by E. coli Cell-free, with N-10*His labeled tag. The total length of MFN2 Protein, Human (Cell-Free, His) is 757 a.a., with molecular weight of 87.9 kDa.

MFN2, a mitochondrial outer membrane GTPase, intricately governs mitochondrial dynamics by mediating both clustering and fusion processes. This GTPase orchestrates the equilibrium between mitochondrial fusion and fission, critically shaping the dynamic morphology of these organelles. Notably, its overexpression induces the formation of extensive mitochondrial networks, emphasizing its pivotal role in mitochondrial organization. The GTPase activity of MFN2 is essential for membrane clustering and may involve significant rearrangements in the coiled coil domains. Beyond its structural contributions, MFN2 emerges as a central player in mitochondrial metabolism, implicating its association with obesity and apoptosis processes. Furthermore, it regulates vascular smooth muscle cell proliferation, demonstrating its diverse roles in cellular functions. In the realm of mitochondrial quality control, MFN2 is instrumental in clearing damaged mitochondria through selective autophagy (mitophagy) by facilitating the recruitment of PRKN to dysfunctional mitochondria. Additionally, it plays a crucial role in modulating the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress, acting as an upstream regulator of EIF2AK3 and suppressing its activation under basal conditions. This multifaceted functionality underscores the significance of MFN2 in cellular homeostasis and stress response mechanisms.

Human

E. coli Cell-free

N-10*His

O95140 (M1-R757)

9927

MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVEGTDGHEAFLLTEGSEEKRSAKTVNQLAHALHQDKQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKHFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRSQAGDRIFFVSAKEVLNARIQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHMAAREQQVYCEEMREERQDRLKFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDDYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAITNSLQTMQQDMIDGLKPLLPVSVRSQIDMLVPRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPIPLTPANPSMPPLPQGSLTQEEFMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR

87.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.