MMP-8 Protein, Human (HEK293, His)

MMP-8, also known as matrix metalloproteinase-8 or collagenase-2, is recognized for its enzymatic abilities, particularly its ability to break down fibrillar types I, II, and III collagen. This enzymatic activity suggests that MMP-8 plays an important role in the remodeling and turnover of collagen-rich tissues such as connective tissue, cartilage, and bone. MMP-8 Protein, Human (HEK293, His) is the recombinant human-derived MMP-8 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of MMP-8 Protein, Human (HEK293, His) is 447 a.a., with molecular weight of approximately 64.09 kDa.

MMP-8, also referred to as matrix metalloproteinase-8 or collagenase-2, is recognized for its enzymatic capabilities and specifically its ability to break down fibrillar type I, II, and III collagens. This enzymatic activity suggests that MMP-8 plays a significant role in the remodeling and turnover of collagen-rich tissues, such as connective tissues, cartilage, and bone. By degrading these fibrillar collagens, MMP-8 can contribute to processes such as tissue remodeling, wound healing, and inflammation resolution. Understanding the precise functions and regulation of MMP-8 can provide valuable insights into its involvement in collagen metabolism and tissue remodeling, potentially offering therapeutic opportunities for conditions characterized by abnormal collagen degradation or accumulation.

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is 8505.224 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-10*His

P22894 (F21-G467)

4317  [NCBI]

FPVSSKEKNTKTVQDYLEKFYQLPSNQYQSTRKNGTNVIVEKLKEMQRFFGLNVTGKPNEETLDMMKKPRCGVPDSGGFMLTPGNPKWERTNLTYRIRNYTPQLSEAEVERAIKDAFELWSVASPLIFTRISQGEADINIAFYQRDHGDNSPFDGPNGILAHAFQPGQGIGGDAHFDAEETWTNTSANYNLFLVAAHEFGHSLGLAHSSDPGALMYPNYAFRETSNYSLPQDDIDGIQAIYGLSSNPIQPTGPSTPKPCDPSLTFDAITTLRGEILFFKDRYFWRRHPQLQRVEMNFISLFWPSLPTGIQAAYEDFDRDLIFLFKGNQYWALSGYDILQGYPKDISNYGFPSSVQAIDAAVFYRSKTYFFVNDQFWRYDNQRQFMEPGYPKSISGAFPGIESKVDAVFQQEHFFHVFSGPRYYAFDLIAQRVTRVARGNKWLNCRYG

approximately 64.09 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.