Moesin Protein, Human (His)

Moesin proteins connect the actin cytoskeleton to the plasma membrane and regulate cell cortical structure and function. Moesin Protein, Human (His) is the recombinant human-derived Moesin protein, expressed by E. coli , with N-His labeled tag. The total length of Moesin Protein, Human (His) is 346 a.a., with molecular weight of ~45 kDa.

Moesin, an Ezrin-radixin-moesin (ERM) family protein, serves as a crucial link between the actin cytoskeleton and the plasma membrane, thereby influencing the structure and function of specific domains in the cell cortex. Functioning as a tether for actin filaments, Moesin undergoes oscillation between a resting and an activated state, establishing transient interactions with the actin cytoskeleton. Upon phosphorylation of its C-terminal threonine, Moesin becomes activated, leading to interactions with F-actin and subsequent cytoskeletal rearrangements. These rearrangements play a pivotal role in regulating various cellular processes, including cell shape determination, membrane transport, and signal transduction. Moesin's significance is particularly pronounced in immunity, impacting both T and B-cell homeostasis and self-tolerance, and regulating lymphocyte egress from lymphoid organs. Additionally, Moesin modulates phagolysosomal biogenesis in macrophages and participates in immunologic synapse formation. The protein adopts a closed conformation (inactive form) through a head-to-tail association of its N-terminal and C-terminal halves, rendering it incapable of actin or membrane-binding in this state.

Human

E. coli

N-His

P26038 (M1-E346)

4478  [NCBI]

Approximately 45 kDa

Greater than 80% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 0.5M NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.