MST2 Protein, Human (Sf9, GST)

MST2 protein is a stress-activated kinase that plays an important role in the Hippo signaling pathway, regulates organ size and inhibits tumors. Cleaved MST2 enters the nucleus and induces apoptosis through chromatin condensation. MST2 Protein, Human (Sf9, GST) is the recombinant human-derived MST2 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of MST2 Protein, Human (Sf9, GST) is 491 a.a., .

MST2 protein, a stress-activated, pro-apoptotic kinase, contributes to various cellular processes, serving as a key component in the Hippo signaling pathway that plays a central role in organ size control and tumor suppression. Upon caspase-cleavage, MST2 enters the nucleus, inducing chromatin condensation and internucleosomal DNA fragmentation, promoting apoptosis. In the Hippo pathway, MST2, along with its counterpart STK4/MST1 and the regulatory protein SAV1, forms a kinase cascade crucial for phosphorylating and activating LATS1/2-MOB1 complex. This cascade further phosphorylates and inactivates the YAP1 oncoprotein and WWTR1/TAZ, regulating genes essential for cell proliferation, death, and migration. MST2 and MST1 are vital for repressing hepatocyte proliferation, preventing activation of facultative adult liver stem cells, and inhibiting tumor formation. Beyond the Hippo pathway, MST2 phosphorylates NKX2-1, NEK2, CROCC, CEP250, MOBKL1A, MOBKL1B, and RASSF2, exerting influence on centrosome disjunction, RAF1 activation, ESR1 transcriptional activity, and STK38 activation. MST2, working in conjunction with SAV1, positively regulates RAF1 activation by suppressing inhibitory phosphorylation on 'Ser-259' and collaborates with MOBKL1B to activate STK38.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The KM of MST2 P3 is 54.85 µM.

Human

Sf9 insect cells

N-GST

Q13188 (M1-F491)

6788

MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF

Approximately 82.8 KDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol 5mM DTT,0.1M Trehalose.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.