MTH1 Protein, Human (His)

The MTH1 protein functions as an oxidative purine nucleoside triphosphohydrolase and is essential for the protection of cellular nucleotide pools. It catalyzes the hydrolysis of oxidized purine nucleoside triphosphates (such as 2-oxo-dATP and 8-oxo-dGTP), preventing their incorporation into DNA and avoiding base pair transversions. MTH1 Protein, Human (His) is the recombinant human-derived MTH1 protein, expressed by E. coli , with N-His labeled tag.

MTH1 Protein functions as an oxidized purine nucleoside triphosphate hydrolase, playing a crucial role in maintaining the integrity of the cellular nucleotide pool. This enzyme catalyzes the hydrolysis of various oxidized purine nucleoside triphosphates, including 2-oxo-dATP, 2-oxo-ATP, 8-oxo-dGTP, and 8-oxo-dATP, preventing their incorporation into DNA and thereby safeguarding against transversions in DNA base pairs. Additionally, MTH1 prevents the integration of methylated purine nucleoside triphosphates into DNA, contributing to its antimutagenic activity and protecting cells from the deleterious effects of oxidative stress. The multifaceted hydrolase activity of MTH1 underscores its significance in preserving genomic stability and mitigating the impact of oxidative damage on cellular processes.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His

P36639-4/NP_002443.3 (M1-V156)

4521  [NCBI]

Approximately 20.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.