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MXRA8 Protein, Mouse (HEK293, His)

Cat. No.: HY-P700877
COA Handling Instructions

MXRA8 is a transmembrane protein that complexly regulates signaling pathways likely by interacting with the integrin ITGAV:ITGB3. It mediates heterophil-cell interactions, inhibits osteoclastogenesis through TNFSF11/RANKL and CSF1, and inhibits VEGF-induced activation of AKT and p38 MAP kinase. MXRA8 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MXRA8 protein, expressed by HEK293 , with C-His labeled tag. The total length of MXRA8 Protein, Mouse (HEK293, His) is 321 a.a., with molecular weight of 42-52 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $210 In-stock
50 μg $405 In-stock
100 μg $650 In-stock
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Description

MXRA8 is a transmembrane protein that complexly regulates signaling pathways likely by interacting with the integrin ITGAV:ITGB3. It mediates heterophil-cell interactions, inhibits osteoclastogenesis through TNFSF11/RANKL and CSF1, and inhibits VEGF-induced activation of AKT and p38 MAP kinase. MXRA8 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MXRA8 protein, expressed by HEK293 , with C-His labeled tag. The total length of MXRA8 Protein, Mouse (HEK293, His) is 321 a.a., with molecular weight of 42-52 kDa.

Background

MXRA8, a transmembrane protein, intricately modulates various signaling pathways, potentially through its interaction with integrin ITGAV:ITGB3. Functionally, MXRA8 mediates heterophilic cell-cell interactions and inhibits osteoclastogenesis downstream of TNFSF11/RANKL and CSF1. This inhibitory effect is suggested to occur by attenuating signaling via integrin ITGB3 and MAP kinase p38. Moreover, MXRA8 plays a crucial role in cartilage formation, promoting the proliferation and maturation of growth plate chondrocytes, stimulating the formation of primary cilia, and enhancing the expression of genes involved in the hedgehog signaling pathway. In angiogenesis, MXRA8 exhibits a dual role by suppressing endothelial cell migration and promoting apoptosis, along with inhibiting VEGF-induced activation of AKT and p38 MAP kinase. Additionally, MXRA8 interferes with VTN-mediated integrin ITGAV:ITGB3 signaling and PTK2/FAK activation. Its involvement extends to the maturation and maintenance of the blood-brain barrier. MXRA8 forms homodimers in cis, and its interaction with ITGB3 inhibits the formation of ITGAV:ITGB3 heterodimers. These diverse functions underscore the multifaceted regulatory role of MXRA8 in cellular processes and tissue homeostasis.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q9DBV4 (V20-Q340)

Gene ID
Molecular Construction
N-term
MXRA8 (V20-Q340)
Accession # Q9DBV4
His
C-term
Synonyms
Adipocyte-specific protein 3; Adipocyte-specific 3; DICAM; Asp3; Limitrin
Molecular Weight

42-52 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

MXRA8 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
MXRA8 Protein, Mouse (HEK293, His)
Cat. No.:
HY-P700877
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