NCKIPSD Protein, Human (His)

NCKIPSD Protein, vital for stress fiber formation induced by active DRF1, stimulates N-WASP-induced ARP2/3 complex activation, independent of CDC42. Implicated in sarcomere maintenance, myofibril assembly, actin polymerization, and angiogenesis. Associates with vimentin, desmin, NCK, GRB2, and interacts with Helicobacter pylori toxin vacA, FHOD1, FASLG, and TMIGD2, showcasing its diverse cellular interactions. NCKIPSD Protein, Human (His) is the recombinant human-derived NCKIPSD protein, expressed by E. coli, with N-His labeled tag. The total length of NCKIPSD Protein, Human (His) is 244 a.a., with molecular weight of ~33-45 kDa.

NCKIPSD Protein plays a crucial role in the formation of stress fibers induced by active diaphanous protein homolog 1 (DRF1) and promotes microspike formation in vivo. It also stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 in vitro. Additionally, it is involved in the maintenance of sarcomeres and the assembly of myofibrils into sarcomeres, contributing to actin polymerization and cell adhesion regulation. NCKIPSD Protein is implicated in angiogenesis and associates with intermediate filaments such as vimentin and desmin. It binds to the SH3 domains of NCK, GRB2, and diaphanous protein homolog 1 (DRF1), as well as the proline-rich domains of N-WASP. It interacts with Helicobacter pylori toxin vacA, FHOD1 (isoform 4), FASLG, and TMIGD2.

Human

E. coli

N-His

Q9NZQ3-3 (M1-T244)

51517  [NCBI]

MYRALYAFRSAEPNALAFAAGETFLVLERSSAHWWLAARARSGETGYVPPAYLRRLQGLEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGPSASSVAVMTSSTSDHHLDAAAARQPNGVCRAGFERQHSLPSSEHLGADGGLYQIPPQPRRAAPTTPPPPVKRRDREALMASGSGGHNTMPSGGNSVSSGSSVSSTSLDTLYTSSSPSEPGSSCSPTPPPVPRRGT

Approximately 33-45 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.