NEDD4L Protein, Human

The NEDD4L protein is an E3 ubiquitin protein ligase that regulates multiple signaling pathways, including autophagy, innate immunity, and DNA repair. NEDD4L Protein, Human is the recombinant human-derived NEDD4L protein, expressed by E. coli , with tag free. The total length of NEDD4L Protein, Human is 974 a.a., .

NEDD4L, an E3 ubiquitin-protein ligase, exerts regulatory control over diverse cellular processes, including autophagy, innate immunity, and DNA repair. It negatively modulates TGF-beta signaling by inducing ubiquitination and proteasome-dependent degradation of SMAD2 and TGFBR1. In the context of autophagy, NEDD4L downregulates cell growth by ubiquitinating and reducing cellular levels of ULK1 or ASCT2. Additionally, it facilitates the ubiquitination and internalization of various plasma membrane channels, such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, Kv7.2, Kv7.3, and CLC5. NEDD4L also regulates the protein levels of SGK1, TNK2, BRAT1, and NTRK1, with its ubiquitin ligase activity influenced by NDFIP1. Furthermore, NEDD4L plays a crucial role in dendrite formation by melanocytes, contributes to TOR signaling regulation, and participates in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, enhancing 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3.

Human

E. coli

Tag Free

Q96PU5 (A2-D975)

23327

The theoretical MW is 111.9 KDa

Greater than 85% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.