1. Recombinant Proteins
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E3 Ligases
  5. NEDD4L Protein, Human

NEDD4L Protein, Human

Cat. No.: HY-P701595
Handling Instructions

The NEDD4L protein is an E3 ubiquitin protein ligase that regulates multiple signaling pathways, including autophagy, innate immunity, and DNA repair. NEDD4L Protein, Human is the recombinant human-derived NEDD4L protein, expressed by E. coli , with tag free. The total length of NEDD4L Protein, Human is 974 a.a., .

For research use only. We do not sell to patients.

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Description

The NEDD4L protein is an E3 ubiquitin protein ligase that regulates multiple signaling pathways, including autophagy, innate immunity, and DNA repair. NEDD4L Protein, Human is the recombinant human-derived NEDD4L protein, expressed by E. coli , with tag free. The total length of NEDD4L Protein, Human is 974 a.a., .

Background

NEDD4L, an E3 ubiquitin-protein ligase, exerts regulatory control over diverse cellular processes, including autophagy, innate immunity, and DNA repair. It negatively modulates TGF-beta signaling by inducing ubiquitination and proteasome-dependent degradation of SMAD2 and TGFBR1. In the context of autophagy, NEDD4L downregulates cell growth by ubiquitinating and reducing cellular levels of ULK1 or ASCT2. Additionally, it facilitates the ubiquitination and internalization of various plasma membrane channels, such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, Kv7.2, Kv7.3, and CLC5. NEDD4L also regulates the protein levels of SGK1, TNK2, BRAT1, and NTRK1, with its ubiquitin ligase activity influenced by NDFIP1. Furthermore, NEDD4L plays a crucial role in dendrite formation by melanocytes, contributes to TOR signaling regulation, and participates in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, enhancing 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q96PU5 (A2-D975)

Gene ID

23327

Molecular Construction
N-term
NEDD4L (A2-D975)
Accession # Q96PU5
C-term
Synonyms
NEDD4L; E3 ubiquitin-protein ligase NEDD4-like; HECT-type E3 ubiquitin transferase NED4L; NEDD4.2; Nedd4-2
Purity

Greater than 85% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NEDD4L Protein, Human
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