NEK9 Protein, Human (Sf9, GST)

The NEK9 protein is a pleiotropic mitotic regulator that oversees spindle dynamics and chromosome segregation. It phosphorylates a variety of substrates, including histones, myelin basic protein, β-casein, and BICD2. NEK9 Protein, Human (Sf9, GST) is the recombinant human-derived NEK9 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of NEK9 Protein, Human (Sf9, GST) is 978 a.a., .

NEK9, a pleiotropic regulator of mitotic progression, orchestrates critical processes in spindle dynamics and chromosome separation. Its pivotal role extends to phosphorylating various substrates, including histones, myelin basic protein, beta-casein, and BICD2. Specifically, NEK9 exhibits phosphorylation activity towards histone H3 on serine and threonine residues and beta-casein on serine residues. The multifaceted influence of NEK9 is underscored by its involvement in the G1/S transition and S phase progression. Moreover, NEK9 plays a key role in modulating the activity of NEK6 and NEK7, enhancing their functionality by releasing the autoinhibitory functions of Tyr-108 and Tyr-97, respectively. This comprehensive regulatory role emphasizes NEK9's significance in governing mitotic processes and ensuring the precise orchestration of cellular events during cell cycle progression.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The KM of NEK9 is 54.69 µM

Human

Sf9 insect cells

N-GST

Q8TD19 (S2-L979);(ΔT346-K731)

91754

Approximately 91.6 KDa

Greater than 80% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH7.5), 200 mM NaCl, 1 mM DTT, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.