Neprilysin/CD10 Protein, Rat (HEK293, His)

Neprilysin/CD10 protein has a specificity similar to thermolysin and mainly acts on polypeptides of up to 30 amino acids. It plays a crucial role in degrading opioid peptides, including Met-enkephalin and Leu-enkephalin, by cleaving the Gly-Phe bond. Neprilysin/CD10 Protein, Rat (HEK293, His) is the recombinant rat-derived Neprilysin/CD10 protein, expressed by HEK293 , with N-His labeled tag. The total length of Neprilysin/CD10 Protein, Rat (HEK293, His) is 699 a.a., with molecular weight of ~93 kDa.

The Neprilysin/CD10 Protein exhibits a thermolysin-like specificity but is primarily active on polypeptides of up to 30 amino acids. Biologically significant, it plays a crucial role in the degradation of opioid peptides, including Met- and Leu-enkephalins, achieved through the cleavage of a Gly-Phe bond. Additionally, Neprilysin/CD10 catalyzes the cleavage of bradykinin, substance P, and neurotensin peptides, and it can cleave angiotensin-1, angiotensin-2, and angiotensin 1-9. The protein is implicated in the degradation of the atrial natriuretic factor (ANF) and exhibits UV-inducible elastase activity towards skin preelastic and elastic fibers, highlighting its diverse enzymatic functions (By similarity).

Measured by its ability to cleave a peptide substrate, Mca-(ala7,lys(dnp)9)-bradykinin. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read). The specific activity is 12527.20 pmoL/min/µg, as measured under the described conditions.

Rat

HEK293

N-His

P07861 (Y52-W750)

24590  [NCBI]

YDDGICKSSDCIKSAARLIQNMDASAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLTLLPDIYGWPVASQNWEQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQRLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEINGKPFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYKEEEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSARQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFTWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW

Approximately 90-95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.