NETO1 Protein, Human (HEK293, His)

The NETO1 protein complexly maintains neuronal circuits as an auxiliary subunit of NMDARs, which is critical for the abundance of GRIN2A-containing NMDARs in the postsynaptic density. It regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, particularly in spatial learning and memory. NETO1 Protein, Human (HEK293, His) is the recombinant human-derived NETO1 protein, expressed by HEK293 , with C-His labeled tag.

The NETO1 Protein is intricately involved in the development and maintenance of neuronal circuitry. Serving as an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR), NETO1 plays a critical role in maintaining the abundance of NMDARs containing GRIN2A in the postsynaptic density. Furthermore, NETO1 functions as a regulator of long-term NMDA receptor-dependent synaptic plasticity and cognition, particularly in the context of spatial learning and memory. The protein's interactions extend to the PLZ domains of DLG2, DLG3, and DLG4 through its C-terminal TRV domain, highlighting its involvement in synaptic signaling complexes. Additionally, NETO1 interacts with GRIN2A and GRIN2B via its CUB domains, indicating its role in modulating the composition and function of NMDARs in neuronal synapses.

Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human Amyloid Precursor binds with an ED₅₀ of 0.6861 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human Amyloid Precursor binds with an ED₅₀ of 0.6861 μg/mL.

Human

HEK293

C-6*His

Q8TDF5-3 (T23-T344)

81832  [NCBI]

TKKGTEKQTTSETQKSVQCGTWTKHAEGGIFTSPNYPSKYPPDRECIYIIEAAPRQCIELYFDEKYSIEPSWECKFDHIEVRDGPFGFSPIIGRFCGQQNPPVIKSSGRFLWIKFFADGELESMGFSARYNFTPDPDFKDLGALKPLPACEFEMGGSEGIVESIQIMKEGKATASEAVDCKWYIRAPPRSKIYLRFLDYEMQNSNECKRNFVAVYDGSSSVEDLKAKFCSTVANDVMLRTGLGVIRMWADEGSRNSRFQMLFTSFQEPPCEGNTFFCHSNMCINNTLVCNGLQNCVYPWDENHCKEKRKTSLLDQLTNTSGT

Approximately 40-50 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.