NLGN1 Protein, Human (HEK293, C-His)

Neuroligin-1/NLGN1 protein is a cell surface protein that is actively involved in synaptic function and transmission by interacting with members of the neuroligin family. Although NLGN1 is not essential for initial synapse formation, it is involved in the formation of de novo presynaptic structures. NLGN1 Protein, Human (HEK293, C-His) is the recombinant human-derived NLGN1 protein, expressed by HEK293 , with C-His labeled tag.

Neuroligin-1 (NLGN1), a cell surface protein, actively participates in cell-cell interactions through its engagement with neurexin family members. It plays a pivotal role in synapse function and the transmission of synaptic signals, likely by recruiting and clustering various synaptic proteins. While NLGN1 may contribute to the initial formation of synapses, it is not deemed indispensable for this process. In vitro studies indicate its ability to initiate the de novo formation of presynaptic structures, suggesting involvement in the specification of excitatory synapses. Moreover, NLGN1 is crucial for maintaining the quality of wakefulness and normal synchrony of cerebral cortex activity during wakefulness and sleep. This protein's intricate interactions include binding to neurexins, specifically NRXN1, NRXN2, and NRXN3, mediated by heparan sulfate glycan modification on neurexin. Additionally, NLGN1 interacts with NLGN3, AIP1, PDZRN3, DLG4/PSD-95 (via PDZ domain 3), and GOPC, collectively contributing to its role in nervous system development.

Measured by its binding ability in a functional ELISA. When recombinant human Neuroligin-1 is immobilized at 5 μg/mL, biotinylated recombinant human Neurexin 1 beta binds with an apparent KD is 36.43 nM.

Human

HEK293

C-His

Q8N2Q7-2/NP_055747.1 (Q46-S677)

22871  [NCBI]

QKLDDVDPLVATNFGKIRGIKKELNNEILGPVIQFLGVPYAAPPTGERRFQPPEPPSPWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLDVVSSYVQDQSEDCLYLNIYVPTEDDIRDSGGPKPVMVYIHGGSYMEGTGNLYDGSVLASYGNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGSGAGGSCVNLLTLSHYSEGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYARMLATKVGCNVSDTVELVECLQKKPYKELVDQDIQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENIVDSDDGISASDFDFAVSNFVDNLYGYPEGKDVLRETIKFMYTDWADRHNPETRRKTLLALFTDHQWVAPAVATADLHSNFGSPTYFYAFYHHCQTDQVPAWADAAHGDEVPYVLGIPMIGPTELFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWTRYSQKDQLYLHIGLKPRVKEHYRANKVNLWLELVPHLHNLNDISQYTSTTTKVPSTDITFRPTRKNSVPVTSAFPTAKQDD

85-95 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.