NGFR Protein, Human (HEK293, Fc)

Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation^[1]. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells^[3]. NGFR Protein, Human (HEK293, Fc) is a recombinant protein with a C-Terminal Fc label, It consists of 250 amino acids (M1-N250) and is produced in HEK293 cells.

Nerve Growth Factor Receptor (NGFR) is expressed not only in nervous tissue, but also in non-neuronal normal and cancer cells, such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells^[1].
Human NGFR shares 92.45% aa sequence identity with mouse NGFR protein and 92.42% aa sequence identity with rat NGFR protein.
Nerve Growth Factor Receptor (NGFR) is a type-I transmembrane protein, a typical structure of the TNFR superfamily and devoid of intrinsic catalytic activity. NGFR signaling involves activation of NF-kB (Rel/NF-kB transcription factors) and the phosphorylation of the transcription factor c-Jun kinase (JNK), as well as increased production of ceramide, leading to gene transcription or programmed cell death^[2].
NGFR induces p53-dependent apoptosis and cell growth arrest as well as suppressed tumor growth^[3]. The low-affinity nerve growth factor receptor (NGFR) p75NGFR induces apoptosis in the absence of nerve growth factor (NGF) binding but enhances neural survival when bound by NGF. NGFR enhances beta-amyloid peptide toxicity^[4]. NGFR signal can induce the subsequent downregulation of melanoma antigens and eventually suppress CTL activation^[5].

NGFR (human) knockdown induces apoptosis and inhibits p53 activation in H46 cells^[3].
NGFR (human) high expression is correlated with greater regional nodal metastasis rates and poor prognosis in human oral squamous cell carcinoma^[6].

Human

HEK293

C-hFc

P08138-1 (K29-N250)

4804  [NCBI]

70-80 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. Reis-Filho JS, et al. Distribution and significance of nerve growth factor receptor (NGFR/p75NTR) in normal, benign and malignant breast tissue. Mod Pathol. 2006 Feb;19(2):307-19.  [Content Brief]

  [2]. Micera A, et al. Nerve growth factor and tissue repair remodeling: trkA(NGFR) and p75(NTR), two receptors one fate. Cytokine Growth Factor Rev. 2007 Jun-Aug;18(3-4):245-56.  [Content Brief]

  [3]. Zhou X, et al. Nerve growth factor receptor negates the tumor suppressor p53 as a feedback regulator. Elife. 2016 Jun 10;5:e15099.  [Content Brief]

  [4]. Rabizadeh S, et al. Expression of the low-affinity nerve growth factor receptor enhances beta-amyloid peptide toxicity. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10703-6.  [Content Brief]

  [5]. Furuta J, et al. CD271 on melanoma cell is an IFN-γ-inducible immunosuppressive factor that mediates downregulation of melanoma antigens. J Invest Dermatol. 2014 May;134(5):1369-1377.  [Content Brief]

  [6]. Chung MK, et al. CD271 Confers an Invasive and Metastatic Phenotype of Head and Neck Squamous Cell Carcinoma through the Upregulation of Slug. Clin Cancer Res. 2018 Feb 1;24(3):674-683.  [Content Brief]