2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. nitA Protein, Rhodococcus rhodochrous (His)

nitA Protein, Rhodococcus rhodochrous (His)

Cat. No.: HY-P703515
Handling Instructions Technical Support

nitA protein enzymatically acts on aliphatic nitriles, specifically acrylonitrile, crotononitrile, and glutaronitrile substrates. nitA Protein, Rhodococcus rhodochrous (His) is the recombinant nitA, expressed by E. coli , with His labeled tag. ,

For research use only. We do not sell to patients.

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Description

nitA protein enzymatically acts on aliphatic nitriles, specifically acrylonitrile, crotononitrile, and glutaronitrile substrates. nitA Protein, Rhodococcus rhodochrous (His) is the recombinant nitA, expressed by E. coli , with His labeled tag. ,
Background

nitA protein demonstrates its enzymatic activity on aliphatic nitriles, specifically targeting substrates like acrylonitrile, crotononitrile, and glutaronitrile.
Species

Others
Source

E. coli
Tag

His
Accession

Q02068 (M1-K369)
Gene ID

/
Synonyms
Aliphatic nitrilase; Rhodococcus rhodochrous; Hydrolase; 3.5.5.7
Molecular Weight

Approximately 43.4 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

nitA Protein, Rhodococcus rhodochrous (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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