NKG2C-CD94 Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi)

NKG2C/CD159c Protein, an immune activating receptor, crucially contributes to self-nonself discrimination. Partnering with KLRD1, it recognizes non-classical MHC class Ib HLA-E loaded with peptides from HLA-G, impacting adaptive NK cell functions and maternal-fetal tolerance. NKG2C/CD159c regulates terminally differentiated cytotoxic lymphocytes, particularly in adaptive NK cell responses to viral infection. Upon binding to HLA-E-peptide complexes, it transmits intracellular signals via TYROBP/DAP12, initiating cell activation. The multifaceted interactions highlight its essential role in immune responses and self-tolerance mechanisms. NKG2C-CD94 Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived NKG2C-CD94 Heterodimer protein, expressed by HEK293, with C-Avi, N-His labeled tag. NKG2C-CD94 Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of 25-35 kDa.

NKG2C/CD159c Protein, an immune activating receptor, plays a pivotal role in self-nonself discrimination. Teaming up with KLRD1 on cytotoxic lymphocyte subsets, it recognizes non-classical major histocompatibility (MHC) class Ib HLA-E loaded with signal sequence-derived peptides from non-classical MHC class Ib HLA-G molecules. This interaction likely contributes to the generation and effector functions of adaptive natural killer (NK) cells and is implicated in maternal-fetal tolerance during pregnancy. NKG2C/CD159c also regulates the effector functions of terminally differentiated cytotoxic lymphocyte subsets, particularly in the adaptive NK cell response to viral infection. Upon binding to HLA-E-peptide complexes, NKG2C/CD159c transmits intracellular signals through the adapter protein TYROBP/DAP12, initiating the phosphorylation of proximal signaling molecules and subsequent cell activation. Forming a heterodimer with KLRD1 through disulfide linkage, the KLRD1-KLRC2 receptor complex, in association with TYROBP homodimers, is crucial for its cell surface expression. Additionally, this receptor complex can bind with low affinity to HLA-E loaded with self-peptides derived from the signal sequence of classical MHC class Ia. The multifaceted interactions and functions of NKG2C/CD159c underscore its essential role in immune responses and self-tolerance mechanisms.

Human

HEK293

N-His;N-Avi

P26717 (E98-L231)&Q13241-1 (S34-I179)

3822&3824

25-35 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.