1. Recombinant Proteins
  2. Receptor Proteins
  3. Notch family
  4. Notch-4
  5. Notch 4 Protein, Mouse (HEK293, His)

Notch 4 Protein, Mouse (HEK293, His)

Cat. No.: HY-P701028
COA Handling Instructions

Notch 4 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and is responsible for coordinating cell fate decisions.Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes in the cleavage site enhancer.Notch 4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Notch 4 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $130 In-stock
50 μg $250 In-stock
100 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

Notch 4 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and is responsible for coordinating cell fate decisions.Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes in the cleavage site enhancer.Notch 4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Notch 4 protein, expressed by HEK293 , with C-His labeled tag.

Background

Notch 4 protein serves as a receptor for membrane-bound ligands, including Jagged1, Jagged2, and Delta1, thereby orchestrating cell-fate determination. Following ligand activation, the released notch intracellular domain (NICD) forms a transcriptional activator complex with RBPJ/RBPSUH, instigating the activation of genes within the enhancer of split locus. This versatile protein influences cellular differentiation, proliferation, and apoptotic programs, potentially playing a role in regulating branching morphogenesis during vascular system development. Structurally, it exists as a heterodimer comprising a C-terminal fragment (N(TM)) and a N-terminal fragment (N(EC)), likely connected by disulfide bonds. Notch 4 interacts with transcriptional coactivators MAML1, MAML2, and MAML3, which further modulate downstream transcriptional processes.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

P31695 (E1042-L1441)

Gene ID
Molecular Construction
N-term
Notch 4 (E1042-L1441)
Accession # P31695
His
C-term
Synonyms
Notch4; Int-3; Int3; Notch-4;
Molecular Weight

50-60 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.22μm filtered solution of PB, 500mM NaCl, 10% glycerol, pH 7.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Notch 4 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Notch 4 Protein, Mouse (HEK293, His)
Cat. No.:
HY-P701028
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