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NOV/CCN3 Protein, Canine (HEK293, His)

Cat. No.: HY-P76520
COA Handling Instructions

NOV/CCN3 Protein belongs to an emerging family of growth regulators referred under the CCN acronym. NOV/CCN3 Protein enables heparin and binding. NOV/CCN3 Protein is involved in cell adhesion and signal transduction. NOV/CCN3 Protein, Canine (HEK293, His) is the recombinant canine-derived NOV/CCN3 protein, expressed by HEK293 , with C-His labeled tag. The total length of NOV/CCN3 Protein, Canine (HEK293, His) is 325 a.a., with molecular weight of 42-50 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

NOV/CCN3 Protein belongs to an emerging family of growth regulators referred under the CCN acronym. NOV/CCN3 Protein enables heparin and binding. NOV/CCN3 Protein is involved in cell adhesion and signal transduction. NOV/CCN3 Protein, Canine (HEK293, His) is the recombinant canine-derived NOV/CCN3 protein, expressed by HEK293 , with C-His labeled tag. The total length of NOV/CCN3 Protein, Canine (HEK293, His) is 325 a.a., with molecular weight of 42-50 KDa.

Background

NOV/CCN3 Protein belongs to an emerging family of growth regulators referred under the CCN acronym. The CCN family now comprises six identified members with properties of both positive and negative regulators of cell growth, sharing a common multimodular organization. NOV/CCN3 Protein enables heparin and binding. NOV/CCN3 Protein is involved in cell adhesion and signal transduction[1][2][3].

Biological Activity

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion, rhNOV, immobilized at 10 µg/mL, will induce 62.85% adhesion on Balbc/3T3 cells (100 µL/well at 3 x 104 cells/mL).

Species

Canine

Source

HEK293

Tag

C-6*His

Accession

J9P4N7 (Q29-M353)

Gene ID
Molecular Construction
N-term
CCN3 (Q29-M353)
Accession # J9P4N7
His
C-term
Synonyms
Nephroblastoma Overexpressed Gene Protein; IBP-9; CCN3; IGFBP9; NOVH
AA Sequence

QRCPTQCPARCAPTPPACAPGVRAVLDDCSCCLVCARQRGESCSPLQPCEESRGLFCDRRADPSAGGGICMAVEGDNCVFDGVIYQSGETFQPSCKYQCACRDGQIGCVPRCGEDLLLPQPDCPAPRKVEVPGECCEKWICDSNEKGELGGLALPAYRTEATLGVAVSDSGINCIEQTTEWSACSKSCGMGFSTRVTNRNPQCEMVKQTRLCMVRPCEQEHQQPADKKGKKCLRTTKSLKAIHLQFKNCTSLHTYKPRFCGVCSDGRCCTPHNTKTIQVEFQCSPGQIIKKPVMVIGTCTCHSNCPHNREAFLQELKPNTSRGEM

Molecular Weight

Approximately 42-50 due to the glycosylation

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

NOV/CCN3 Protein, Canine (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NOV/CCN3 Protein, Canine (HEK293, His)
Cat. No.:
HY-P76520
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